| 项目编号 | PRJCA006162 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 项目标题 | Transcriptome and translatome analyses of mouse and human oocytes | ||||||||
| 涉及领域 | Medical | ||||||||
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads |
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| 物种名称 |
Mus musculus
Homo sapiens |
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| 描述信息 | Translatomes are more accurate than transcriptomes as indicators of gene expression profiles in cells, especially in cellular processes that cease transcriptional expression and rely on translational control, such as oocytes. Here, we developed a dual omics methodology to simultaneously examine gene expression using the transcriptomes and translatomes of single oocytes or embryos and identified a protein that can promote human oocyte maturation. First, we developed a transcriptome and translatome sequencing (T&T seq) strategy using single mouse oocytes and extended this method to human oocytes and early embryos. Many genes that are differentially expressed through translational control at different developmental stages and between two species were identified using this method. Overall, our dual omics methodology opens a new avenue for simultaneously assessing the transcriptome and translatome of a cell. | ||||||||
| 样品范围 | Multispecies | ||||||||
| 发布日期 | 2023-08-13 | ||||||||
| 项目资金来源 |
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| 提交者 | Kehkooi Kee (kkee@tsinghua.edu.cn) | ||||||||
| 提交单位 | Tsinghua University | ||||||||
| 提交日期 | 2021-08-13 |