| 项目编号 |
PRJCA006185 |
| 项目标题 |
Functional characterization of UDP-glycosyltransferases involved in anti-viral lignan glycosides biosynthesis in Isatis indigotica |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Isatis tinctoria
|
| 描述信息 |
Isatis indigotica has garnered attention in recent years for its antiviral properties,. While several studies have analyzed enzymatic genes during the biosynthesis of lignan glycosides in I. indigotica, the key dirigent protein (DIR) forming pinoresinol and glycosyltransferase, which is involved in the production of these two lignan glycosides, have yet to be identified. In this study, we characterized three glycosyltransferases that transformed lariciresinol into lariciresinlucosides in vitro from ten candidate IiUGT genes. Analysis of transgenic hairy roots demonstrated that IiUGT10 primarily participates in the biosynthesis of coniferin and that IiUGT1 primarily participates in the biosynthesis of pinoresinol diglucoside, while IiUGT4 could be responsible for the glycosylation of lariciresinol and play a dominant role in the biosynthesis of lariciresinol glycosides in I. indigotica. Analysis of the molecular docking and site-mutagenesis of IiUGT4 have found that key residues for its catalytic activity are H373, W376, E397, and that F151 could be associated with substrate preference. This study elucidates the biosynthetic route of antiviral lignan glycosides, which breaks ground for the production of anti-viral lignan glycosides via synthetic biology under the heterologous model. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2021-08-16 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 35774804
|
Functional Characterization of UDP-Glycosyltransferases Involved in Anti-viral Lignan Glycosides Biosynthesis in Isatis indigotica
|
Frontiers in Plant Science
|
10.3389/fpls.2022.921815
|
2022
|
|
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| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| No funding support
|
|
|
|
|
|
| 提交者 |
Tong
Chen (chentong_biology@163.com)
|
| 提交单位 |
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences |
| 提交日期 |
2021-08-16 |
