| 项目编号 |
PRJCA006374 |
| 项目标题 |
Single-molecular-based 16S rDNA taxonomy approach to identify the microbiota alternation in vaginal tract response to the treatment of metronidazole |
| 涉及领域 |
Medical |
| 数据类型 |
Metagenome
|
| 物种名称 |
human vaginal metagenome
|
| 描述信息 |
Human genital tract colonialized with a diverse array of beneficial microbes and opportunistic pathogens. The dysbiosis of vaginal ecosystem contributes to the overgrowth of pathogens which causes complicated vaginal infections such as bacterial vaginosis (BV). Metronidazole is the first-line therapeutic drug to suppress BV-associated bacteria and alleviate symptoms. Despite of capturing the heterogeneity of microbes for bulk methods, the greatest recent advancements in our understanding of complex organisms has happened in the field of single-cell genetic analysis. Here, we adopted single-molecular-based 16S rDNA taxonomy approach to identify the microbiota alternation in vaginal tract response to the treatment of metronidazole, and revealed over 15% of unexplored Lactobacillus. Importantly, most of Lactobacillus were not significantly increased but L.iners, which suggested the important roles of Metronidazole treatment. Our findings provide the importance of the single-cell paradigm for progressing the microbiology field and applying these insights to better understand of BV treatments. |
| 样品范围 |
Environment |
| 发布日期 |
2021-09-13 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 37199621
|
Single-Molecule Approach to 16S rRNA for Vaginal Microbiome Signatures in Response to Metronidazole Treatment
|
Microbiology Spectrum
|
10.1128/spectrum.01706-22
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
No.81971342
|
|
| National Natural Science Foundation of China (NSFC)
|
|
No.81200411
|
|
|
|
| 提交者 |
Bingbing
Xiao (doctorxbb@163.com)
|
| 提交单位 |
Peking University First Hospital |
| 提交日期 |
2021-08-31 |
