| 项目编号 |
PRJCA006720 |
| 项目标题 |
Proteomic Analysis of the Development of Haemaphysalis longicornis based on the iTRAQ Technique |
| 涉及领域 |
Agricultural |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Haemaphysalis longicornis
|
| 描述信息 |
Ticks are important parasites that cause more diseases than most other animal parasites. Haemaphysalis longicornis is used as an experimental animal model for the study of three-host ticks due to its special life cycle and easy maintenance in the laboratory and in its reproduction. The life cycle of H. longicornis goes through a tightly regulated life cycle to adapt to the changing host and environment, and these stages of transition are also accompanied by proteome changes in the body. In this study, the aim was to use the isobaric tags for relative and absolute quantification (iTRAQ) technique to systematically describe and analyze the dynamic expression of protein and the molecular basis of the proteome of H. longicornis in seven differential developmental stages (eggs, unfed larvae, fed larvae, unfed nymphs, fed nymphs unfed adults, and fed adults). A total of 2,059 proteins were identified, and their expression profiles were classified at different developmental stages. In addition, it was found that tissue and organ development-related proteins and metabolism-related proteins showed that they were involved in different physiological processes throughout the life cycle through the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the differentially expressed proteins (DEPs). More importantly, we found that the upregulated proteins of fed adult ticks were mainly related to yolk absorption, degradation, and ovarian development-related proteins. The abundance of the cuticle proteins in the unfed stages were significantly higher compared with those of the fed ticks in the previous stages. In short, the protein spectrum changes identified in this study provide a reference proteome for future studies of tick functional proteins and provide candidate targets for elucidating tick development and developing new tick control strategies. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2021-09-27 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
|
|
Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of Haemaphysalis Longicornis (Parthenogenesis)
|
Research Square
|
10.21203/rs.3.rs-1213265/v1
|
2022
|
| 39859999
|
Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of Haemaphysalis longicornis (Parthenogenesis)
|
Life
|
10.3390/life15010059
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
2019YFC1200502
|
Discovery of new pathogens carried by important vertebrate vectors and their potential pathogenicity
|
|
|
| 提交者 |
liu
wenge (466948677@qq.com)
|
| 提交单位 |
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou, China |
| 提交日期 |
2021-09-27 |
