| Accession | PRJCA006785 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Title | Nuclear translocation of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation | ||||||||||
| Relevance | Medical | ||||||||||
| Data types |
Epigenomics
Raw sequence reads Genome sequencing |
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| Organisms | Mus musculus | ||||||||||
| Description | Pluripotent stem cells hold great promise in regenerative medicine and developmental biology studies. Mitochondrial metabolites, including tricarboxylic acid (TCA) cycle intermediates, have been reported to play critical roles in pluripotency acquisition and transition. Here we show that enzymes of the TCA cycle, including Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha and Mdh2, are translocated to the nucleus during pluripotency acquisition and transition. The translocated TCA cycle enzymes, particularly nuclear targeted Pdha1, could promote pluripotency acquisition and transition. Mechanistically, nuclear Phda1 increases the acetyl-CoA pool in the nucleus, leading to chromatin remodeling at pluripotency genes by enhancing histone H3 acetylation. Our results reveal an important role of mitochondrial TCA cycle enzymes in the epigenetic regulation of pluripotency that constitutes a novel mitochondria-to-nucleus retrograde signaling model in pluripotency acquisition and transition. | ||||||||||
| Sample scope | Monoisolate | ||||||||||
| Release date | 2022-11-13 | ||||||||||
| Publication |
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| Grants |
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| Submitter | Li Wei (huitu09@163.com) | ||||||||||
| Organization | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences | ||||||||||
| Submission date | 2021-10-04 |
| Resource name | Description |
|---|---|
| BioSample (53) show | - |
| GSA (3) | - |
| CRA005144 | Nuclear translocation of mitochondrial TCA cycle enzymes modulates pluripotency |
| CRA005143 | Nuclear translocation of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation |
| CRA007453 | Nuclear translocation of mitochondrial TCA cycle enzymes modulates somatic cell reprogramming |