| Accession |
PRJCA006868 |
| Title |
mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation |
| Relevance |
Medical |
| Data types |
Proteomics
|
| Organisms |
Mus musculus
|
| Description |
Using mouse models, we explored the role of RNA binding proteins in regulation of the biological activities of this population. Proteins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured with oligo (dT) conjugated beads after UV crosslinking and profiled by proteomics (termed mRBPome capture), yielding a putative repertoire of 473 RBPs. From this database, the RBP TRIM71 was identified and found to be expressed by stem and progenitor spermatogonia in prepubertal and adult mouse testes. Tissue specific deletion of TRIM71 in the male germline led to reduction of the undifferentiated spermatogonial population and a block in transition to the differentiating state. Collectively, these findings demonstrate a key role of the RBP system in regulation of the spermatogenic lineage and may provide clues about the influence of RBPs on the biology of progenitor cell populations in other lineages. |
| Sample scope |
Monoisolate |
| Release date |
2021-11-15 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 32188631
|
|
|
|
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2018YFC1003302
|
|
|
|
| Submitter |
Lufan
Li (lilufan@njmu.edu.cn)
|
| Organization |
Nanjing Medical University |
| Submission date |
2021-10-14 |
