| Description |
Bisphenol A (BPA), a currently widely used chemical in the production of plastics and resins, has long been recognized with male reproductive toxicities. A comprehensive investigation of cell-type specific responses and cellular heterogeneity upon BPA-associated testicular injury especially at the prepubertal stage is required to comprehensive provide precise molecular and cellular pathways for understanding its toxicity. Accordingly, we performed single-cell transcriptome analysis of 77776 cells of testicular tissue from untreated and BPA (10 mg/kd-bw. d from postnatal day 22-43) treated C57BL/6 mice. Our results show comprehensive information of differential expressed genes, pathways, transcriptional factors (TFs), and ligand-receptors in major testis cell types upon BPA-treatment in testis. We identified shared and cell type specific gene signatures, pathways, TFs, and receptor-ligand pairs in testis, and highlight key molecular processes including RNA splicing, steroid metabolism, spermatid differentiation, ER stress underlying BPA-induced perpetual injury. Overall, our high-resolution cellular atlas not only provide novel insight into underlying mechanisms and pathways for BPA-associated testicular injury, but also represents a valuable resource and foundation of information to additional discoveries and modifying BPA-induced male reproduction toxicity in mice. |
