| Accession |
PRJCA007628 |
| Title |
PAC5 shows potent activity against HBV and CoV in vivo |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
proteome and transcriptome
|
| Organisms |
Homo sapiens
|
| Description |
Viral infection is a major global health burden for which novel immunotherapeutic approaches are needed. Herein, we synthesized a novel sesquiterpene derivative PAC5 that suppressed hepatitis B virus (HBV) infection in vivo and in vitro with no toxicity. The strategy of activity-based protein profilings (ABPPs) was used to explore the functional target(s) of PAC5. We synthesized both a positive probe (PAC5-bio, PP) and a negative probe (PAC3-bio, NP) from PAC5 and PAC3 (an inactive analogue of PAC5), respectively, with biotin and an ethylene glycol linker. Then, we performed three independent pull-down experiments using positive and negative probes, respectively. Peptide mass fingerprinting data analysis identified specific proteins in the positive probe pulldowns. RNAseq was also used to explore the effect and action mechanism of PAC5 in HepG2.2.15 cells |
| Sample scope |
Monoisolate |
| Release date |
2021-12-19 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 36726760
|
A hnRNPA2B1 agonist effectively inhibits HBV and SARS-CoV-2 omicron in vivo
|
Protein & Cell
|
10.1093/procel/pwac027
|
2023
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
31960093
|
|
|
|
| Submitter |
shaoxing
Dai (daishaoxing@163.com)
|
| Organization |
Institute of Primate Translational Medicine, Kunming University of Science and Technology |
| Submission date |
2021-12-19 |
