| 项目编号 |
PRJCA007628 |
| 项目标题 |
PAC5 shows potent activity against HBV and CoV in vivo |
| 涉及领域 |
Medical |
| 数据类型 |
Transcriptome or Gene expression
proteome and transcriptome
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Viral infection is a major global health burden for which novel immunotherapeutic approaches are needed. Herein, we synthesized a novel sesquiterpene derivative PAC5 that suppressed hepatitis B virus (HBV) infection in vivo and in vitro with no toxicity. The strategy of activity-based protein profilings (ABPPs) was used to explore the functional target(s) of PAC5. We synthesized both a positive probe (PAC5-bio, PP) and a negative probe (PAC3-bio, NP) from PAC5 and PAC3 (an inactive analogue of PAC5), respectively, with biotin and an ethylene glycol linker. Then, we performed three independent pull-down experiments using positive and negative probes, respectively. Peptide mass fingerprinting data analysis identified specific proteins in the positive probe pulldowns. RNAseq was also used to explore the effect and action mechanism of PAC5 in HepG2.2.15 cells |
| 样品范围 |
Monoisolate |
| 发布日期 |
2021-12-19 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 36726760
|
A hnRNPA2B1 agonist effectively inhibits HBV and SARS-CoV-2 omicron in vivo
|
Protein & Cell
|
10.1093/procel/pwac027
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
General Program
|
31960093
|
|
|
|
| 提交者 |
shaoxing
Dai (daishaoxing@163.com)
|
| 提交单位 |
Institute of Primate Translational Medicine, Kunming University of Science and Technology |
| 提交日期 |
2021-12-19 |
