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项目编号 PRJCA007795
项目标题 Single-Nucleus Profiling Identifies Microglia Subsets Exacerbating Motor Neuron Aging in Primate Spinal Cord
涉及领域 Model organism
数据类型 Raw sequence reads
Single cell sequencing
物种名称 Macaca fascicularis
Homo sapiens
描述信息 The spinal cord is an extension of the central nervous system connecting brain and peripheral nerves. Degeneration of spinal cord links to impairment of motor, sensory, and autonomic function, and occurrence of aging-related myelopathy. Yet, compared to brain, spinal cord is rarely studied, and a systematic delineation of the molecular signature in aged spinal cord is still unexplored. Here, we conducted histological and molecular analysis, as well as transcriptomic profiling at both bulk-tissue and single-nucleus levels, to depict the molecular landscape of primate spinal cord aging. Besides motor neuron loss, dendritic defect, accumulation of oxidative stress, we identified elevated neuroinflammation as a prominent feature of aged spinal cord. Moreover, we characterized an unappreciated spinal cord localized aging-related microglia (sc-ARM) subset exhibiting a high pro-inflammatory signature. CHIT1, one of the top upregulated genes detected at bulk level, is exclusively expressed in sc-ARM, accumulated in aged plasma, making it an ideal diagnostic marker for spinal cord aging. Moreover, sc-ARM accumulated in the proximity of senescent motor neuron in aged spinal cord and treatment with CHIT1 triggered senescence of human motor neuron which could be abrogated by Vitamin C treatment. Altogether, our findings depict the first transcriptomic atlas of the aged primate spinal cord and provide vital insights into the key biomarker and driver for spinal cord degeneration.
样品范围 Multispecies
发布日期 2023-10-18
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
39920442 Multiomics identify the gene expression signature of the spinal cord during aging process Communications Biology 10.1038/s42003-025-07475-4 2025
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
No funding support
提交者 Guanghui Liu (ghliu@ioz.ac.cn)
提交单位 Institute of Zoology, Chinese Academy of Sciences
提交日期 2022-01-02

项目包含数据信息

资源名称 描述
BioSample (24)  show -
GSA (1) -
CRA005773 CHIT1-positive microglia drive motor neuron aging in the primate spinal cord