| 项目编号 |
PRJCA007996 |
| 项目标题 |
Nucleosome reconfiguration in mouse somatic cell nuclear transfer embryos |
| 涉及领域 |
Model organism |
| 数据类型 |
Raw sequence reads
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Somatic cell nuclear transfer (SCNT) can reprogram terminally differentiated somatic cells into totipotent embryos. However, the birth rate of SCNT embryos is extremely low compared with that of fertilized embryos. But the underlying molecular mechanisms remain unclear. The nucleosome, which is the fundamental structural unit of chromatin in eukaryotes, is closely related to gene expression. How nucleosome position change after the donor cell nuclear transferred into enucleated oocytes is largely unexplored. Here, we mapped genome-wide profiling of nucleosome occupancy and positioning in early mouse SCNT embryos using ultra-low-input MNase-seq (ULI-MNase-seq). We observed the nucleosome depletion regions (NDRs) around TSS regions disappeared as early as 1 hour after the nuclear injection and re-established until 6 hours after activation of the SCNT embryos, which indicated nucleosome position undergo dramatic changes in early SCNT embryos. Dynamic of nucleosome position occurred in SCNT embryos were different from those in fertilized embryos. Subsequently, we defined differentially expressed genes between donor cumulus cells and SCNT or fertilized embryos at ICM stage, and found the gene expression levels of ICM SCNT embryos was positively correlated with the NDR scores around TSS regions in donor cumulus cells. The results indicated that aberrant nucleosome occupancy at promoters and enhancers in donor cumulus cells was a potential cause of developmental defects of SCNT embryos. Finally, we found that the genes with high nucleosome occupancy at promoters was regulated by histone acetylation to rebuild NDRs. Our study provide insight into nucleosome reconfiguration during early SCNT embryo development. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2022-05-27 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2021YFA1102900
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2020YFA0112500
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32070802
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32000418
|
|
| China Postdoctoral Science Foundation
|
|
2021M692437
|
|
|
|
| 提交者 |
xiaocui
xu (x18810404054@126.com)
|
| 提交单位 |
Tongji University |
| 提交日期 |
2022-01-18 |
