Accession PRJCA008215
Title CRY2 interacts with CIS1 to regulate alternative splicing and thermosensory flowering
Relevance Agricultural
Data types Transcriptome or Gene expression
Raw sequence reads
Organisms Arabidopsis thaliana
Description Cryptochromes (CRYs) are evolutionarily conserved photolyase-like photoreceptors found in almost all species, including mammals. CRYs regulate transcription by modulating the activity of several transcription factors, but whether and how they affect pre-mRNA processing are unknown. Photoperiod and temperature are closely associated seasonal cues that influence reproductive timing in plants. CRYs mediate photoperiod-responsive floral initiation, but it is largely unknown whether and how they are also involved in thermosensory flowering. We establish here that blue light and CRY2 play critical roles in thermosensory flowering in Arabidopsis thaliana by regulating RNA alternative splicing (AS) to affect protein expression and development. CRY2 INTERACTING SPLICING FACTOR 1 (CIS1) interacts with CRY2 in a blue light-dependent manner and promotes thermosensory flowering in a CRY2-dependent manner. Blue light, CRYs, and CISs affect transcriptome-wide AS profiles, including those of FLOWERING LOCUS M (FLM), which is critical for temperature modulation of flowering. Moreover, CIS1 binds to the FLM pre-mRNA to regulate its AS, while CRY2 regulates the RNA-binding activity of CIS1. Thus, blue light regulates thermosensory flowering via a CRY2-CIS1-FLM signaling pathway that links flowering responses to both light and ambient temperature.
Sample scope Multiisolate
Release date 2024-02-10
Agency program Grant ID Grant title
Chinese Academy of Sciences (CAS) XDB27030000
Submitter Zhiwei    Zhao  (
Organization CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology
Submission date 2022-02-10

Project Data

Resource name Description
BioSample (18)  show -
GSA (1) -
CRA006040 Next Generation Sequencing Facilitates Quantitative Analysis of WT and cry1cry2 mutant and cis1-1 mutant in dark and Blue light Transcriptomes