Accession

PRJCA008495

Title

Single cell and spatial transcriptome revealed tumor heterogeneity and their close relation with systematic immune environment in breast cancer

Relevance

Medical

Data types

Single cell sequencing

Organisms

Homo sapiens

Description

Tumor heterogeneity plays essential roles in developing cancer therapies, including BC. It is also very important to understand the relationships between tumor microenvironments and systematic immune environment. Here we performed single cell and spatial transcriptome analysis on tumor and adjacent normal tissue as well as axillar lymph nodes (LNs) and peripheral blood mononuclear cells (PBMC). We found that myeloid cells exhibited environment dependent plasticity where a group of macrophages with both M1 and M2 signatures possessed high tumor specificity and predicted patient survivals. Cytotoxic T cells in tumor sites evolved in a separated path from those in circulating system, while helper and regulatory T cells diverged respectively in circulating system before infiltrating into tumor. Immune environment in metastasis LN was significant different from that in non-metastasis LN, but similar to that in tumor microenvironment. Cells interactions showed that cancer associated fibroblasts contributed most in shaping immune suppressive microenvironment, while CD8 cells were the most signal responding cells. This study revealed cell structures of both micro and macro environment, exhibited how different cells diverged in related contexts as well as their capacities of prognosis, and provided useful information in developing targeted and immune therapies.

Sample scope

Single cell

Release date

2022-09-07

Publication

PubMed ID	Article title	Journal name	DOI	Year
36040519	Single-cell and spatial transcriptome analyses revealed cell heterogeneity and immune environment alternations in metastatic axillary lymph nodes in breast cancer	Cancer immunology, immunotherapy	10.1007/s00262-022-03278-2	2022

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)	Major Program	81972335
National Natural Science Foundation of China (NSFC)	Young Scientists Fund	82103347

Submitter

Wei Luo (luowei_421@163.com)

Organization

The first people's hospital of Foshan

Submission date

2022-02-28

Project Data

Resource name	Description
BioSample (22) show	-
SAMC2023798	CD45+ sorting cells from Tumor tissue of Breast cancer patient
SAMC2023795	CD45+ sorting cells from Breast normal tissue of Breast cancer patient
SAMC2023789	CD45+ sorting cells from Tumor tissue of Breast cancer patient
SAMC2023786	CD45+ sorting cells from Tumor tissue of Breast cancer patient
SAMC2023783	CD45+ sorting cells from Tumor tissue of Breast cancer patient
SAMC2023779	Total cells from PBMC of Breast cancer patient
SAMC2023775	Total cells from Breast tumor of Breast cancer patient
SAMC2023771	Total cells from Breast normal tissue of Breast cancer patient
SAMC2023767	Total cells from axillary lymph node of Breast cancer patient
SAMC2023764	Total cells from PBMC of Breast cancer patient
SAMC2023760	Total cells from Breast tumor of Breast cancer patient
SAMC2023757	Total cells from Breast normal tissue of Breast cancer patient
SAMC2023753	Total cells from axillary lymph node of Breast cancer patient
SAMC2023750	Total cells from PBMC of Breast cancer patient
SAMC2023746	Total cells from Breast tumor of Breast cancer patient
SAMC2023742	Total cells from Breast normal tissue of Breast cancer patient
SAMC2023738	Total cells from axillary lymph node of Breast cancer patient
SAMC2023734	Total cells from PBMC of Breast cancer patient
SAMC2023730	Total cells from Breast tumor of Breast cancer patient
SAMC2023727	Total cells from Breast normal tissue of Breast cancer patient
SAMC2023723	Total cells from axillary lymph node of Breast cancer patient
SAMC2023720	Total cells from axillary lymph node of Breast cancer patient