Accession |
PRJCA008530 |
Title |
Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and contamination identification methods |
Relevance |
Medical |
Data types |
Metagenome
Raw sequence reads
|
Organisms |
synthetic construct
Bacteria
|
Description |
To obtain high-quality metagenomic libraries and high-fidelity data from sub-nanogram DNA, we evaluated the performance of various library preparation methods on 0.5 pg - 5 ng synthetic microbial community DNA, characterized contaminants and further applied different in silico decontamination methods. |
Sample scope |
Synthetic |
Release date |
2022-09-23 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
36209213
|
Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methods
|
BMC Biology
|
10.1186/s12915-022-01418-9
|
2022
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
Beijing Nova Program
|
|
Z211100002121135
|
|
|
Submitter |
Li
Mingkun (limk@big.ac.cn)
|
Organization |
Beijing Institute of Genomics, Chinese Academy of Sciences |
Submission date |
2022-03-03 |