| Accession |
PRJCA008591 |
| Title |
The peripheral blood microbiome analysis via NIPT reveals the complexity of circulating microbial cell-free DNA baselines |
| Relevance |
Medical |
| Data types |
Metagenome
|
| Organisms |
root
|
| Description |
Noninvasive prenatal testing (NIPT) has emerged as a valuable tool in the early detection of fetal chromosomal anomalies in pregnant women, and accordingly cell-free circulating DNA (cfDNA) is becoming a powerful liquid biopsy marker for noninvasive diagnosis of diseases, especially in identifying infectious pathogens. Currently, detecting the microbe cfDNA pattern or baselines in health individuals is urgently needed for the proper interpretation of microbe cfDNA sequencing results in clinical settings. Here we utilized the standard low-pass whole genome sequencing-based NIPT to establish the microbial cfDNA baseline in health people. Totally 107,763 sequencing data from pregnant women peripheral blood samples who underwent NIPT screening were collected and re-analyzed for microbiome DNA screening. Overall and regional abundance patterns were illustrated, and normal baselines for individual microorganism were estimated and evaluated together with model parameters potentially useful for other sequencing schemes. The unique inter-species and symbiosis relationship was also established with regional features. It was found that the prevalence of major viruses showed great diversity in different regions. To sum up, our study revealed the complexity of circulating microbial cfDNA baselines, and the interpretation of microbial cfDNA sequencing results should be performed in a more comprehensive manner. |
| Sample scope |
Multispecies |
| Release date |
2022-04-01 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 35608350
|
Peripheral Blood Microbiome Analysis via Noninvasive Prenatal Testing Reveals the Complexity of Circulating Microbial Cell-Free DNA
|
Microbiology Spectrum
|
10.1128/spectrum.00414-22
|
2022
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Key Research and Development Program of China
|
|
2018YFC2000505
|
|
| National Key Research and Development Program of China
|
|
2020YFC2009003
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2021-I2M-1-050
|
|
| Applied Research Program of Capital Clinical Features
|
|
Z18110001718172
|
|
| National Natural Science Foundation of China
|
|
81870013
|
|
| Natural Science Foundation of Beijing
|
|
7202178
|
|
|
|
| Submitter |
Yang
Du (yangdu@annoroad.com)
|
| Organization |
Annoroad Gene Technology |
| Submission date |
2022-03-09 |
