| 项目编号 |
PRJCA008591 |
| 项目标题 |
The peripheral blood microbiome analysis via NIPT reveals the complexity of circulating microbial cell-free DNA baselines |
| 涉及领域 |
Medical |
| 数据类型 |
Metagenome
|
| 物种名称 |
Homo sapiens
human blood metagenome
|
| 描述信息 |
Noninvasive prenatal testing (NIPT) has emerged as a valuable tool in the early detection of fetal chromosomal anomalies in pregnant women, and accordingly cell-free circulating DNA (cfDNA) is becoming a powerful liquid biopsy marker for noninvasive diagnosis of diseases, especially in identifying infectious pathogens. Currently, detecting the microbe cfDNA pattern or baselines in health individuals is urgently needed for the proper interpretation of microbe cfDNA sequencing results in clinical settings. Here we utilized the standard low-pass whole genome sequencing-based NIPT to establish the microbial cfDNA baseline in health people. Totally 107,763 sequencing data from pregnant women peripheral blood samples who underwent NIPT screening were collected and re-analyzed for microbiome DNA screening. Overall and regional abundance patterns were illustrated, and normal baselines for individual microorganism were estimated and evaluated together with model parameters potentially useful for other sequencing schemes. The unique inter-species and symbiosis relationship was also established with regional features. It was found that the prevalence of major viruses showed great diversity in different regions. To sum up, our study revealed the complexity of circulating microbial cfDNA baselines, and the interpretation of microbial cfDNA sequencing results should be performed in a more comprehensive manner. |
| 样品范围 |
Multispecies |
| 发布日期 |
2022-04-01 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 35608350
|
Peripheral Blood Microbiome Analysis via Noninvasive Prenatal Testing Reveals the Complexity of Circulating Microbial Cell-Free DNA
|
Microbiology Spectrum
|
10.1128/spectrum.00414-22
|
2022
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Key Research and Development Program of China
|
|
2018YFC2000505
|
|
| National Key Research and Development Program of China
|
|
2020YFC2009003
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2021-I2M-1-050
|
|
| Applied Research Program of Capital Clinical Features
|
|
Z18110001718172
|
|
| National Natural Science Foundation of China
|
|
81870013
|
|
| Natural Science Foundation of Beijing
|
|
7202178
|
|
|
|
| 提交者 |
Yang
Du (yangdu@annoroad.com)
|
| 提交单位 |
Annoroad Gene Technology |
| 提交日期 |
2022-03-09 |
