项目编号

PRJCA008779

项目标题

scm6A-seq reveals the epitranscriptomic landscape during oocyte maturation and early embryo differentiation of mouse

涉及领域

Model organism

数据类型

Raw sequence reads

物种名称

Mus musculus

描述信息

We employ RNA multiplex-labeling methods and SMART (witching mechanism at the 5' end of RNA template) technology to improve the method to profile the m6A genome widely from single cells, named single-cell m6A-seq (scm6A-seq). scm6A-seq sequence the gene expression level with m6A information, making a robust tool to classify cell types more accurately. Our research reveals the dynamic m6A landscape and roles during early mammalian embryo development. Providing a more sophisticated approach to study the epitranscriptome during oocyte maturation and embryogenesis at single cell level.

样品范围

Single cell

发布日期

2024-10-09

出版信息

PubMed ID	文章标题	杂志名称	Doi	发表年份
36658155	scm⁶A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development	Nature Communications	10.1038/s41467-023-35958-7	2023

项目资金来源

机构	项目类型	授权项目ID
National Natural Science Foundation of China (NSFC)	Science Fund for Creative Research Groups	32121001
Chinese Academy of Sciences (CAS)	Strategic Priority Research Program of Chinese Academy of Sciences (CAS)	XDA16010501
Chinese Academy of Sciences (CAS)	Strategic Priority Research Program of Chinese Academy of Sciences (CAS)	XDB38020500
Chinese Academy of Sciences (CAS)	Strategic Priority Research Program of Chinese Academy of Sciences (CAS)	XDPB2004
CAS Project for Young Scientists in Basic Research		YSBR-073
National Key R&D Program of China		2019YFA0110900
National Key R&D Program of China		2019YFA0110901
National Key R&D Program of China		2019YFA0802201
National Natural Science Foundation of China (NSFC)		92053115
National Natural Science Foundation of China (NSFC)		91940304
CAS Key Research Projects of the Frontier Science		QYZDY-SSW-SMC027
Youth Innovation Promotion Association of CAS		2018133
Beijing Nova Program		Z201100006820104
Beijing Nova Program		20220484210
Shanghai Municipal Science and Technology Major Project, China		2017SHZDZX01

提交者

Chun Chun Gao (gaochunchun@big.ac.cn)

提交单位

Beijing Institute of Genomics, Chinese Academy of Sciences

提交日期

2022-03-21

项目包含数据信息

资源名称	描述
BioSample (19) show	-
SAMC1010498	Total single cell RNA-seq of wild type and Mettl3 cKO zygotes
SAMC1010497	Total single cell RNA-seq of wild type and Mettl3 cKO MII oocytes
SAMC690890	Bulk MeRIP-seq of 70 GVs
SAMC690889	Bulk RNA-seq of 70 GVs
SAMC690888	Bulk MeRIP-seq of 50 GVs
SAMC690887	Bulk RNA-seq of 50 GVs
SAMC690886	Bulk MeRIP-seq of 25 GVs
SAMC690885	Bulk RNA-seq of 25 GVs
SAMC690878	m6A-IP of zygote to 4-cell scm6A-seq
SAMC690877	Supernatant of zygote to 4-cell scm6A-seq
SAMC690876	RNA of zygote to 4-cell scm6A-seq
SAMC690875	m6A-IP of GV-GVBD-MI-MII scm6A-seq
SAMC690874	Supernatant of GV-GVBD-MI-MII scm6A-seq
SAMC690873	RNA of GV-GVBD-MI-MII scm6A-seq
SAMC690872	m6A-IP of GV wild-type and Mettl3 cKO scm6A-seq
SAMC690871	Supernatant of GV wild-type and Mettl3 cKO scm6A-seq
SAMC690870	RNA of GV wild-type and Mettl3 cKO scm6A-seq
SAMC690869	m6A-IP of GV scm6A-seq
SAMC690868	RNA of GV scm6A-seq
GSA (1)	-
CRA006425	scm6A-seq reveals the epitranscriptomic landscape during oocyte maturation and early embryo development of mouse