| Accession |
PRJCA008853 |
| Title |
Identifying SARS-CoV-2-specific TCRs and their epitopes of long-term memory T cells 7-8 months after the infection of COVID-19 |
| Relevance |
Medical |
| Data types |
Single cell sequencing
|
| Organisms |
Homo sapiens
|
| Description |
Long-term T cell responses are crucial for efficient protection against SARS-CoV-2 variants. Although T cell epitopes were identified, the exact sequences of the cross-reactive TCRs are unknown. Here, we predicted relatively conserved T cell epitopes among SARS-CoV-2, SARS-COV and other five coronaviruses. Then, to evaluate the long-term T cell immunity, we collected peripheral blood mononuclear cells (PBMCs) from 18 recovered donors 7-8 months after the infection of COVID-19. Predicted epitopes were constructed into tandem minigenes (TMGs) and transfected into engineered K562 cells expressing a single HLA allele. After the co-culture of PBMCs and K562 cells, T cells were analyzed and sorted for single-cell transcriptome profiling coupled with TCR sequencing. With single-cell information, we constructed 34 TCR-engineered T cells as candidates for recognizing SARS-CoV-2 antigens and 19 of them turned out to be responsive to a specific epitope. Furthermore, many of the TCRs hold cross-reactivity to mutant antigens with comparable levels of activation, providing potential protection to SARS-CoV-2 variants. |
| Sample scope |
Single cell |
| Release date |
2024-01-01 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 39496850
|
Identification of virus epitopes and reactive T-cell receptors from memory T cells without peptide synthesis
|
Communications Biology
|
10.1038/s42003-024-07048-x
|
2024
|
|
|
| Biomaterial provider |
Chongqing Medical University |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| China Postdoctoral Science Foundation
|
|
2020T130061ZX
|
|
|
|
| Submitter |
Xun
Lan (xlan@tsinghua.edu.cn)
|
| Organization |
Tsinghua University |
| Submission date |
2022-03-27 |
