| 项目编号 |
PRJCA008925 |
| 项目标题 |
Engineered Phage |
| 涉及领域 |
Mircobiology |
| 数据类型 |
Metagenome
Targeted Locus (Loci)
|
| 物种名称 |
mouse metagenome
|
| 描述信息 |
Bacteriophages (phages) are ideal alternatives to traditional antimicrobial agents in a world where antimicrobial resistance (AMR) is emerging and spreading with an unprecedent speed. We engineered a lambda phage (Eλ) to target enterohemorrhagic Escherichia coli (EHEC) that causes a severe, sometimes lethal intestinal infection in humans. We enhanced the killing ability of the Eλ phage by incorporating a CRISPR-Cas3 system into the wild-type λ (wtλ), and the specificity by introducing multiple EHEC-targeting CRISPR-spacers while knocking out the lytic gene cro. |
| 样品范围 |
Environment |
| 发布日期 |
2022-04-01 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 35876591
|
An Engineered λ Phage Enables Enhanced and Strain-Specific Killing of Enterohemorrhagic Escherichia coli
|
Microbiology Spectrum
|
10.1128/spectrum.01271-22
|
2022
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
32070660
|
|
| NNSF-VR Sino-Swedish Joint Research Programme
|
|
82161138017
|
|
| National Key Research and Development Program of China
|
|
2019YFA0905600
|
|
|
|
| 提交者 |
Wei-Hua
Chen (weihuachen@hust.edu.cn)
|
| 提交单位 |
Huazhong University of Science and Technology |
| 提交日期 |
2022-04-01 |
