| 项目编号 |
PRJCA009196 |
| 项目标题 |
A novel sustainable mouse karyotype created by programmed chromosome fusion |
| 涉及领域 |
Model organism |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
Genome sequencing
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Here we report a novel laboratory mouse model derived by engineering that has fewer chromosome pairs than its wild-type counterpart. The engineering was initially performed within embryonic stem cells carrying a simplified haploid karyotype, in which nonhomologous chromosome arms could be ligated simply after near-ending DNA cleavages. By differentiating haploid stem cells into diploid somatic cells, we found an upper-length-limit for ligated chromosome arms, exceeding which would result in cell polyploidization. By oocyte injection of haploid stem cells, full-term pups carrying ligated chromosomes with arm sizes beneath the upper-length-limit were derived, which could transmit ligated chromosomes to their offspring. Our work achieves chromosome-level rearrangements common in evolution and disease in house mouse, and exhibits a feasible route for performing large-scale DNA engineering in mammals. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2022-08-26 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Chinese Academy of Sciences (CAS)
|
|
XDA16030400
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2019YFA0110800
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2019YFA0903800
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2017YFA0103803
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2018YFA0108400
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31621004
|
|
| Chinese Academy of Sciences (CAS)
|
|
YSBR-012
|
|
| Ministry of Human Resources and Social Security of the People's Republic of China
|
|
BX201600161
|
|
| Ministry of Human Resources and Social Security of the People's Republic of China
|
|
BX201700243
|
|
|
|
| 提交者 |
Libin
Wang (wlbfmb@126.com)
|
| 提交单位 |
Institute of Zoology, Chinese Academy of Sciences |
| 提交日期 |
2022-04-18 |
