| 描述信息 |
As an important clinical pathogen among Enterobacteriaceae, Klebsiella pneumoniae can cause community and hospital-acquired infections, including Ventilator-associated pneumonia (VAP) and invasive pyogenic liver abscess (PLA) with life-threatening malignant consequences commonly occurring, but the underlying pathogenic mechanisms of these two types of virulent K.pneumoniae strains and their differences have not been detailedly clarified. Here, The clinical data, genome sequencing, antimicrobial resistance (AMR) and virulence profile analysis, the hypermucoviscous phenotype, and core genome multilocus sequence typing (cgMLST) of clinical K. pneumoniae isolates from two groups were comprehensively investigated in this study. We then used the Kleberate tool as a predictive model to effectively screen the key biomarker between the two groups. Through a systematic analytical study, we found only limited differences in the biological characteristics, genome, resistance and virulence profiles, and metabolic pathways between VAP and PLA isolates. In terms of epidemiological results, the PLA group had certain underlying disease preferences, especially diabetes and cholelithiasis. The drug resistance results of VAP were significantly higher than that of PLA, while the virulence comparison was opposite. Kleberate tool could well display the AMR and virulence factors of clinical isolates from two groups. The combination of iucA, rmpA, hypermucoviscous phenotype, and ST23 presented in K. pneumoniae strain infection is of more concern and will be beneficial for timely antibiotic treatment. The study could provide a reference for clinical screening, epidemiological surveillance, optimization of patient care, and reasonable antibacterial therapy. |
