Accession PRJCA009533
Title Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression
Relevance Medical
Data types Transcriptome or Gene expression
Organisms Sus scrofa
Description In summary, we have for the first time generated a Dox inducible and switchable tdTomato pig strain with dual knock-in at Hipp11 and Rosa26 locus. This pig model provide a versatile tool model to produce stable transgenic pigs with inducible overexpressing any gene of interest by using the technology of phiC31 integrase-mediated cassette exchange. Based on this approach, an inducible hKRASG12D-expressing pig model line was established and tumor formation was induced after Dox administration. We expect that the inducible tool pig model would greatly facilitate the production of controllable transgenic pigs, and broaden the applications of transgenic pigs in biomedicine and agriculture fields.
Sample scope Bama/Taihu pig
Release date 2022-05-11
Publication
PubMed ID Article title Journal name DOI Year
Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression SCIENCE CHINA Life Sciences 10.1007/s11427-021-2088-1 2022
Grants
Agency program Grant ID Grant title
Chinese Academy of Sciences (CAS) 2017YFA05103
Submitter Qin    Jin  (sciencedb@gibh.ac.cn)
Organization Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Submission date 2022-05-11

Project Data

Resource name Description
BioSample (18)  show -
GSA (1) -
CRA006893 Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression