| Accession |
PRJCA009533 |
| Title |
Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
|
| Organisms |
Sus scrofa
|
| Description |
In summary, we have for the first time generated a Dox inducible and switchable tdTomato pig strain with dual knock-in at Hipp11 and Rosa26 locus. This pig model provide a versatile tool model to produce stable transgenic pigs with inducible overexpressing any gene of interest by using the technology of phiC31 integrase-mediated cassette exchange. Based on this approach, an inducible hKRASG12D-expressing pig model line was established and tumor formation was induced after Dox administration. We expect that the inducible tool pig model would greatly facilitate the production of controllable transgenic pigs, and broaden the applications of transgenic pigs in biomedicine and agriculture fields. |
| Sample scope |
Bama/Taihu pig |
| Release date |
2022-05-11 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
|
|
Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression
|
SCIENCE CHINA Life Sciences
|
10.1007/s11427-021-2088-1
|
2022
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Chinese Academy of Sciences (CAS)
|
|
2017YFA05103
|
|
|
|
| Submitter |
Qin
Jin (sciencedb@gibh.ac.cn)
|
| Organization |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission date |
2022-05-11 |
