| 项目编号 |
PRJCA009560 |
| 项目标题 |
Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation |
| 涉及领域 |
Medical |
| 数据类型 |
Targeted Locus (Loci)
|
| 物种名称 |
Sus scrofa
|
| 描述信息 |
In this study, through the precise knock-in of the binary Tet-On elements, rtTA-expressing cassette and TRE-controlled SpCas9-T2A-tdTomato cassette,, into the Rosa26 and Hipp11 loci, respectively, we attempted to generate a Dox-inducible Cas9-expressing (DIC) pig model that allows flexible temporal control of SpCas9 activity in pigs by simple chemical induction in vitro and in vivo. With this pig model, in vitro and/or in vivo gene editing and chromosome engineering for loss of function and in vitro regulation of endogenous target gene for gain of function could be realized by simply delivering the sgRNAs or transcriptional activation complexes combined with modified dead guide RNAs into porcine fetal fibroblasts (PFFs) and/or specific tissues and following Dox induction. |
| 样品范围 |
Tibetan/Bama pig |
| 发布日期 |
2022-05-12 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 36650523
|
Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation
|
Genome Biology
|
10.1186/s13059-023-02851-x
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| Chinese Academy of Sciences (CAS)
|
|
32170542
|
|
|
|
| 提交者 |
Qin
Jin (sciencedb@gibh.ac.cn)
|
| 提交单位 |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| 提交日期 |
2022-05-12 |
