| Accession |
PRJCA009560 |
| Title |
Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation |
| Relevance |
Medical |
| Data types |
Targeted Locus (Loci)
|
| Organisms |
Sus scrofa
|
| Description |
In this study, through the precise knock-in of the binary Tet-On elements, rtTA-expressing cassette and TRE-controlled SpCas9-T2A-tdTomato cassette,, into the Rosa26 and Hipp11 loci, respectively, we attempted to generate a Dox-inducible Cas9-expressing (DIC) pig model that allows flexible temporal control of SpCas9 activity in pigs by simple chemical induction in vitro and in vivo. With this pig model, in vitro and/or in vivo gene editing and chromosome engineering for loss of function and in vitro regulation of endogenous target gene for gain of function could be realized by simply delivering the sgRNAs or transcriptional activation complexes combined with modified dead guide RNAs into porcine fetal fibroblasts (PFFs) and/or specific tissues and following Dox induction. |
| Sample scope |
Tibetan/Bama pig |
| Release date |
2022-05-12 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 36650523
|
Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation
|
Genome Biology
|
10.1186/s13059-023-02851-x
|
2023
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Chinese Academy of Sciences (CAS)
|
|
32170542
|
|
|
|
| Submitter |
Qin
Jin (sciencedb@gibh.ac.cn)
|
| Organization |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission date |
2022-05-12 |
