Accession PRJCA009560
Title Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation
Relevance Medical
Data types Targeted Locus (Loci)
Organisms Sus scrofa
Description In this study, through the precise knock-in of the binary Tet-On elements, rtTA-expressing cassette and TRE-controlled SpCas9-T2A-tdTomato cassette,, into the Rosa26 and Hipp11 loci, respectively, we attempted to generate a Dox-inducible Cas9-expressing (DIC) pig model that allows flexible temporal control of SpCas9 activity in pigs by simple chemical induction in vitro and in vivo. With this pig model, in vitro and/or in vivo gene editing and chromosome engineering for loss of function and in vitro regulation of endogenous target gene for gain of function could be realized by simply delivering the sgRNAs or transcriptional activation complexes combined with modified dead guide RNAs into porcine fetal fibroblasts (PFFs) and/or specific tissues and following Dox induction.
Sample scope Tibetan/Bama pig
Release date 2022-05-12
Grants
Agency program Grant ID Grant title
Chinese Academy of Sciences (CAS) 32170542
Submitter Qin    Jin  (sciencedb@gibh.ac.cn)
Organization Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Submission date 2022-05-12

Project Data

Resource name Description
BioSample (131)  show -
GSA (1) -
CRA006896 Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation