| 描述信息 |
The cleavage under targets and tagmentation (CUT&Tag) is a new technology to map chromatin protein binding sites. Compared to the chromatin immunoprecipitation with sequencing (ChIP-seq), the CUT&Tag technology was faster, fewer starting material needed, and showed a higher-resolution, lower-background signal than did ChIP. However, few CUT&Tag protocols were developed that were suitable for plants, because of the barriers of the cell wall. So far, no report about the application of CUT&Tag in identification of plant transcription factor (TF) binding sites has been published. In this study, to genome-wide identify the binding sites of ZmMYC2a and ZmMYC2b, which are the key TFs of the jasmonate pathway in maize, we performed CUT&Tag with ZmMYC2a, 2b-eGFP fused proteins overexpressed maize protoplasts. |
