| 项目编号 | PRJCA009859 | ||||||||||
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| 项目标题 | Activation of P53 pathway contributes to Xenopus hybrid inviability | ||||||||||
| 涉及领域 | Model organism | ||||||||||
| 数据类型 |
Whole genome sequencing
Raw sequence reads Genome sequencing |
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| 物种名称 |
Xenopus
Xenopus laevis Xenopus tropicalis |
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| 描述信息 | Incompatibility between the cytoplasm of Xenopus tropicalis eggs and the nucleus of Xenopus laevis sperm (te x ls) leads to specific loss of the majority of paternal chromosomes 3L and 4L. The hybrids die before gastrulation, of which the lethal causes remain unclear. Combining genetic and genomic approaches, we found that in stage 9 embryos, the most enriched motif in upregulated ATAC-seq peaks of te x ls hybrids in comparison with wild-type Xenopus tropicalis (xt.WT) controls was P53 binding motif, which was confirmed by upregulation of P53 target genes in RNA-seq data. Of note, expression levels of tp53 transcripts remained stable in te x ls hybrids, as well as in le x ts hybrids or xt.WT and xl.WT controls. In contrast, P53 protein levels in texls hybrids weakly increased at stage 8 and drastically increased at stage 9, which was not observed in xt.WT and xl.WT controls or le x ts hybrids. Using a tp53 knockout line established in our laboratory, we found that tetp53-/- x ls hybrids can smoothly go through gastrulation and survive till stage 31, indicating a clear rescue that significantly postponed the lethal stage. Overexpression of dominant-negative P53 or Mdm2, a negative regulator of P53, by mRNA injection can also rescue the early lethal of te x ls hybrids to neurula stage of development. Combination of P53 ChIP-seq and ATAC-seq data revealed severe inhibition of tRNA transcription. Together, our data demonstrate that it is the molecular conflicts-activated P53 pathway that contributes to Xenopus te x ls hybrid inviability. | ||||||||||
| 样品范围 | Multiisolate | ||||||||||
| 发布日期 | 2023-05-06 | ||||||||||
| 出版信息 |
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| 项目资金来源 |
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| 提交者 | Hao Jiang (john-jh@foxmail.com) | ||||||||||
| 提交单位 | Southern University of Science and Technology | ||||||||||
| 提交日期 | 2022-05-29 |