| 项目编号 |
PRJCA009927 |
| 项目标题 |
Mechanisms of Estrogen Deficiency-induced Osteoporosis Based on Transcriptome and DNA Methylation |
| 涉及领域 |
Medical |
| 数据类型 |
Whole genome sequencing
Epigenomics
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
Osteoporosis is a disease that impacts the elderly. Reduced estrogen has been related to changes in DNA methylation and consequent alterations in gene expression in a number of studies, leading to a new direction for research into the pathophysiology of osteoporosis. As a consequence, the OVX mouse model was constructed in our study, and it was found that the mouse models were indeed osteoporosis by studying the phenotype and methylation level in the mouse's bone, and at the same time, the methylation level of the OVX mice were significantly changed compared to that of SHAM mice. As a consequence, we performed genome-level analysis on the mouse model using transcriptome and WGBS. We discovered that the changes in gene expression level caused by osteoporosis mainly focus on the decrease of bone and muscle development and the activation of the immune system by combining the data of two omics. These differentially expressed genes and pathways are consistent with the differentially expressed methylation locations and regions, according to intersection analysis of methylation and transcriptome data. We found that the differentially expressed methylation sites of these genes are mainly concentrated in promoters, Exons, and other important functional regions by selecting important differentially expressed genes. This is also the primary cause of gene differential expression variations, indicating that estrogen deficiency might further regulate gene expression by altering methylation modification, leading to the development of osteoporosis. At the same time, the clinical value of methylation modification research is demonstrated in this research. The findings are expected to add to current understanding of the molecular mechanisms underlying osteoporosis incidence and development, as well as provide new ideas for early detection and treatment of osteoporosis. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2022-08-01 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39833331
|
Transcriptome analysis of liver injury of fatty liver disease induced by ALDH2 deficiency
|
Scientific Reports
|
10.1038/s41598-025-86547-1
|
2025
|
| 36325359
|
Mechanisms of estrogen deficiency-induced osteoporosis based on transcriptome and DNA methylation
|
Frontiers in Cell and Developmental Biology
|
10.3389/fcell.2022.1011725
|
2022
|
| 40624035
|
Integrated bioinformatics analysis identifies CHAD association with osteoporosis and in vitro chondrogenic effects of Wogonin
|
Scientific Reports
|
10.1038/s41598-025-05861-w
|
2025
|
| 39617773
|
DNMT aberration-incurred GPX4 suppression prompts osteoblast ferroptosis and osteoporosis
|
Bone Research
|
10.1038/s41413-024-00365-1
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation for Key Programs of China Grants
|
|
82130065
|
|
| National Natural Science Foundation of China
|
|
82002330
|
|
| FENG foundation
|
|
FFBR202103
|
|
| Research Foundation of Capital Institute of Pediatrics
|
|
CXYJ-2021-04
|
|
| Young Elite Scientist Sponsorship Program by the China Association for Science and Technology
|
|
2020-JCJQ-QT-033
|
|
|
|
| 提交者 |
Xu
Ziying (xuziying326@126.com)
|
| 提交单位 |
Capital Institute of Pediatrics |
| 提交日期 |
2022-05-17 |
