| 项目编号 |
PRJCA010387 |
| 项目标题 |
RNA-seq and scRNA-seq of mouse embryos with wild type and Pak2 knock out |
| 涉及领域 |
Model organism |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
Single cell sequencing
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
To investigate how PAK2 affected neural tube closure by regulating gene expression, RNA-seq was performed on WT (3 embryos in one replicate, 3 replicates) and Pak2 knock out (3 embryos in one replicate, 2 replicates) bulk embryos at E9.5. To clarify cell-specific abnormality in the Pak2 knock out embryo, we performed scRNA-seq by using the DNBelab C4 platform. A total of ~48 000 cells that passed quality control in WT and Pak2 knock out embryo were divided into seventeen clusters with t-distributed stochastic neighbor embedding (tSNE). |
| 样品范围 |
Single cell |
| 发布日期 |
2024-07-04 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 36504449
|
Loss-of-Function of p21-Activated Kinase 2 Links BMP Signaling to Neural Tube Patterning Defects
|
Advanced Science
|
10.1002/advs.202204018
|
2022
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China
|
|
32070590
|
|
| National Natural Science Foundation of China
|
|
31871191
|
|
| Guangdong Key Project in the "Development of new tools for diagnosis and treatment of autism"
|
|
2018B030335001
|
|
| a Guangzhou Key Project in "Early diagnosis and treatment of autism spectrum disorders"
|
|
202007030002
|
|
| Strategic Priority Research Program of Chinese Academy of Sciences
|
|
XDPB16
|
|
|
|
| 提交者 |
Zhongsheng
Sun (sunzs@biols.ac.cn)
|
| 提交单位 |
Chinese Academy of Sciences |
| 提交日期 |
2022-07-04 |
