| Accession |
PRJCA010637 |
| Title |
GCTOF-MS Combined LC-QTRAP-MS/MS Reveals Metabolic Difference Between Osteoarthritis and Osteoporotic Osteoarthritis and the Intervention Effect of Erxian Decoction |
| Relevance |
Medical |
| Data types |
metabolism
|
| Organisms |
Rattus
|
| Description |
Purpose: OP and OA are chronic bone diseases with high incidence in the middle-aged and elderly populations. The latest research shows that the pathological environment of OP may be involved in the aggravation of the pathological process of OA, and the pathological state of OP plays an important role in the aggravation of OA pathology. EXD is a traditional Chinese medicine decoction that has been used to treat osteoporosis. Therefore, we further study whether OA will be aggravated in the OP environment and whether EXD can alleviate OA by intervening in the OP environment. The purpose of this study was to analyze the effect of OP on OA metabolites by using metabolomic methods and to explore the intervention mechanism of EXD on osteoporotic OA.Method: Thirty-two SD rats were randomly divided into normal group, OA group, OP-OA group, and EXD group. EXD was administered by gavage. Histopathological evaluation of cartilage tissue was performed using Saffron fast green and HE staining. Western blot and qRT-PCR were used to detect the expression levels of chondrogenesis genes SOX9, COL2A1, and COMP in cartilage tissue. GC-TOFMS and LC-QTRAP-MS/MS metabolomics methods were used to analyze the changes of metabolites in serum samples of rats in each group. |
| Sample scope |
rats blood serum |
| Release date |
2022-07-22 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
|
|
|
|
10.3389/fendo.2022.905507
|
|
| 35966099
|
GCTOF-MS Combined LC-QTRAP-MS/MS Reveals Metabolic Difference Between Osteoarthritis and Osteoporotic Osteoarthritis and the Intervention Effect of Erxian Decoction
|
Frontiers in Endocrinology
|
10.3389/fendo.2022.905507
|
2022
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine
|
|
y2021rc02
|
the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine
|
| the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine
|
|
y2021rc20
|
|
| the General Project of Natural Fund of Nanjing University of Traditional Chinese Medicine
|
|
k2021x05
|
|
|
|
| Submitter |
Zhenyuan
Ma (906459075@qq.com)
|
| Organization |
Affiliated Hospital of Nanjing University of Chinese Medicine |
| Submission date |
2022-07-19 |
