| 项目编号 |
PRJCA010637 |
| 项目标题 |
GCTOF-MS Combined LC-QTRAP-MS/MS Reveals Metabolic Difference Between Osteoarthritis and Osteoporotic Osteoarthritis and the Intervention Effect of Erxian Decoction |
| 涉及领域 |
Medical |
| 数据类型 |
metabolism
|
| 物种名称 |
Rattus
|
| 描述信息 |
Purpose: OP and OA are chronic bone diseases with high incidence in the middle-aged and elderly populations. The latest research shows that the pathological environment of OP may be involved in the aggravation of the pathological process of OA, and the pathological state of OP plays an important role in the aggravation of OA pathology. EXD is a traditional Chinese medicine decoction that has been used to treat osteoporosis. Therefore, we further study whether OA will be aggravated in the OP environment and whether EXD can alleviate OA by intervening in the OP environment. The purpose of this study was to analyze the effect of OP on OA metabolites by using metabolomic methods and to explore the intervention mechanism of EXD on osteoporotic OA.Method: Thirty-two SD rats were randomly divided into normal group, OA group, OP-OA group, and EXD group. EXD was administered by gavage. Histopathological evaluation of cartilage tissue was performed using Saffron fast green and HE staining. Western blot and qRT-PCR were used to detect the expression levels of chondrogenesis genes SOX9, COL2A1, and COMP in cartilage tissue. GC-TOFMS and LC-QTRAP-MS/MS metabolomics methods were used to analyze the changes of metabolites in serum samples of rats in each group. |
| 样品范围 |
rats blood serum |
| 发布日期 |
2022-07-22 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine
|
|
y2021rc02
|
the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine
|
| the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine
|
|
y2021rc20
|
|
| the General Project of Natural Fund of Nanjing University of Traditional Chinese Medicine
|
|
k2021x05
|
|
|
|
| 提交者 |
Zhenyuan
Ma (906459075@qq.com)
|
| 提交单位 |
Affiliated Hospital of Nanjing University of Chinese Medicine |
| 提交日期 |
2022-07-19 |
