| 项目编号 |
PRJCA011155 |
| 项目标题 |
Targeting 16S rDNA with third-generation sequencing improves pathogen identification in bacterial pneumonia |
| 涉及领域 |
Medical |
| 数据类型 |
Metagenome
Genome sequencing
|
| 物种名称 |
Bacteria
|
| 描述信息 |
We developed a target 16S rDNA amplification-based TGS test to identify pathogens. Twenty-one common bacteria from pulmonary infections and 94 BALF samples from severe or prolonged pneumonia patients from the Children's Hospital of Zhejiang University School of Medicine were collected between December 15, 2021, and January 15, 2022. The atTGS test was performed on-site, and the results of low abundance pathogenic bacteria were verified with qPCR. The results were compared with those of routine microbiology tests. |
| 样品范围 |
Multispecies |
| 发布日期 |
2022-08-17 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 36699719
|
Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children
|
Frontiers in Cellular and Infection Microbiology
|
10.3389/fcimb.2022.1001607
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
81901989
|
|
| Zhejiang Provincial Natural Science Foundation of China
|
|
LY21H150005
|
|
|
|
| 提交者 |
Wu
310000
Sihao (407194301@qq.com)
|
| 提交单位 |
Joint Research Center for Molecular Diagnosis of Severe Infection in Children, Binjiang Institute of Zhejiang University, Hangzhou |
| 提交日期 |
2022-08-12 |
