Accession PRJCA011155
Title Targeting 16S rDNA with third-generation sequencing improves pathogen identification in bacterial pneumonia
Relevance Medical
Data types Metagenome
Genome sequencing
Organisms Bacteria
Description We developed a target 16S rDNA amplification-based TGS test to identify pathogens. Twenty-one common bacteria from pulmonary infections and 94 BALF samples from severe or prolonged pneumonia patients from the Children's Hospital of Zhejiang University School of Medicine were collected between December 15, 2021, and January 15, 2022. The atTGS test was performed on-site, and the results of low abundance pathogenic bacteria were verified with qPCR. The results were compared with those of routine microbiology tests.
Sample scope Multispecies
Release date 2022-08-17
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 81901989
Zhejiang Provincial Natural Science Foundation of China LY21H150005
Submitter Wu  310000  Sihao  (407194301@qq.com)
Organization Joint Research Center for Molecular Diagnosis of Severe Infection in Children, Binjiang Institute of Zhejiang University, Hangzhou
Submission date 2022-08-12

Project Data

Resource name Description
BioSample (94)  show -
GSA (1) -
CRA007814 Targeting 16S rDNA with third-generation sequencing improves pathogen identification in bacterial pneumonia