Accession

PRJCA011706

Title

Genetic and evolutionary basis for the formation of plant traits

Relevance

Agricultural

Data types

Whole genome sequencing
Transcriptome or Gene expression
Genome sequencing and assembly
Raw sequence reads
Genome sequencing
Assembly

Organisms

Zea mays

Description

Teosinte, the wild ancestor of maize (Zea mays subsp. mays), has three times the seed protein content of most modern inbreds and hybrids, but the mechanisms that are responsible for this trait are unknown1,2. Here we use trio binning to create a contiguous haplotype DNA sequence of a teosinte (Zea mays subsp. parviglumis) and, through map-based cloning, identify a major high-protein quantitative trait locus, TEOSINTE HIGH PROTEIN 9 (THP9), on chromosome 9. THP9 encodes an asparagine synthetase 4 enzyme that is highly expressed in teosinte, but not in the B73 inbred, in which a deletion in the tenth intron of THP9-B73 causes incorrect splicing of THP9-B73 transcripts. Transgenic expression of THP9-teosinte in B73 significantly increased the seed protein content. Introgression of THP9-teosinte into modern maize inbreds and hybrids greatly enhanced the accumulation of free amino acids, especially asparagine, throughout the plant, and increased seed protein content without affecting yield. THP9-teosinte seems to increase nitrogen-use efficiency, which is important for promoting a high yield under low-nitrogen conditions.

Sample scope

Monoisolate

Release date

2024-04-16

Publication

PubMed ID	Article title	Journal name	DOI	Year
36385527	THP9 enhances seed protein content and nitrogen-use efficiency in maize	Nature	10.1038/s41586-022-05441-2	2022

Grants

Agency	program	Grant ID	Grant title
Chinese Academy of Sciences (CAS)		XDB27010000

Submitter

Qiong Wang (wangqiong@cemps.ac.cn)

Organization

CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology

Submission date

2022-09-06

Project Data

Resource name	Description
BioSample (30) show	-
SAMC874387	Reseq data of Ames21814
SAMC874386	Reseq data of F1 (B73 X Ames21814 )
SAMC874385	HiFi data of F1 (B73 X Ames21814 )
SAMC874357	F1 tassel RNA-seq
SAMC874356	F1 stem RNA-seq
SAMC874355	F1 seed RNA-seq
SAMC874354	F1 root RNA-seq
SAMC874353	F1 leaf RNA-seq
SAMC874352	F1 corncob RNA-seq
SAMC874351	Teo root RNA-seq
SAMC874350	Teo root RNA-seq
SAMC874349	Teo root RNA-seq
SAMC874348	F1 root RNA-seq
SAMC874347	F1 root RNA-seq
SAMC874346	F1 root RNA-seq
SAMC874345	B73 root RNA-seq
SAMC874344	B73 root RNA-seq
SAMC874343	B73 root RNA-seq
SAMC874342	Teo leaf RNA-seq
SAMC874341	Teo leaf RNA-seq
SAMC874340	Teo leaf RNA-seq
SAMC874339	F1 leaf RNA-seq
SAMC874338	F1 leaf RNA-seq
SAMC874337	F1 leaf RNA-seq
SAMC874336	B73 leaf RNA-seq
SAMC874335	B73 leaf RNA-seq
SAMC874334	B73 leaf RNA-seq
SAMC873394	Genome assemble of Zea mays ssp. Parviglumis
SAMC873393	Iso sequencing F1 from Zea mays ssp. Parviglumis and B73
SAMC873392	HiC sequencing F1 from Zea mays ssp. Parviglumis and B73
GSA (3)	-
CRA008106	RNA-seq
CRA008100	Teosinte high protein 9 enhances the seed protein content and NUE in maize
CRA008107	Assemble data