Accession PRJCA012201
Title Decoding Cortical Glial Cell Development
Relevance Evolution
Data types Single cell sequencing
Organisms Mus
Description Mouse cortical radial glial cells (RGCs) are primary neural stem cells that give rise to cortical oligodendrocytes, astrocytes, and olfactory bulb (OB) GABAergic interneurons in late embryogenesis. There are fundamental gaps in understanding how these diverse cell subtypes are generated. Here, by combining single-cell RNA-Seq with intersectional lineage analyses, we show that beginning at around E16.5, neocortical RGCs start to generate ASCL1+EGFR+ apical multipotent intermediate progenitors (MIPCs), which then differentiate into basal MIPCs that express ASCL1, EGFR, OLIG2, and MKI67. These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons. Finally, single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons. Taken together, this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.
Sample scope Single cell
Release date 2022-09-28
Publication
PubMed ID Article title Journal name DOI Year
33606177 Decoding Cortical Glial Cell Development Neurosci Bull. 10.1007/s12264-021-00640-9 2021
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2018YFA0108000
Submitter Zhengang Yang (yangz@fudan.edu.cn)
Organization Fudan University
Submission date 2022-09-28

Project Data

Resource name Description