| Accession |
PRJCA012474 |
| Title |
A human embryonic limb cell atlas resolved in space and time |
| Relevance |
Medical |
| Data types |
Single cell sequencing
|
| Organisms |
Homo sapiens
|
| Description |
Human limbs emerge during the fourth post-conception week as mesenchymal buds which develop into fully-formed limbs over the subsequent months. Limb development is orchestrated by numerous temporally and spatially restricted gene expression programmes, making congenital alterations in phenotype common. Decades of work with model organisms has outlined the fundamental processes underlying vertebrate limb development, but an in-depth characterisation of this process in humans has yet to be performed. Here we detail the development of the human embryonic limb across space and time, using both single-cell and spatial transcriptomics. Finally, we perform scRNA-seq on murine embryonic limbs to facilitate cross-species developmental comparison at single-cell resolution, finding substantial homology between the two species. |
| Sample scope |
Single cell |
| Release date |
2024-10-11 |
| Publication |
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2019YFA0801703
|
|
| National Natural Science Foundation of China (NSFC)
|
General Program
|
31871370
|
|
| Science and Technology Program of Guangzhou
|
|
202002030429
|
|
| China Postdoctoral Science Foundation
|
|
2021M700936
|
|
| Natural Science Foundation of Guangdong
|
|
2019A1515011342
|
|
|
|
| Submitter |
Hongbo
Zhang (zhanghongbo@mail.sysu.edu.cn)
|
| Organization |
Sun Yat-sen University |
| Submission date |
2022-10-13 |
