| 项目编号 |
PRJCA013022 |
| 项目标题 |
A spatiotemporal gene expression and cell atlas of the developing rat ovary |
| 涉及领域 |
Model organism |
| 数据类型 |
Transcriptome or Gene expression
Raw sequence reads
Single cell sequencing
|
| 物种名称 |
Rattus norvegicus
|
| 描述信息 |
Normal ovarian development is necessary for the production of healthy oocytes. However, the characteristics of oocytes development at different stages and the regulatory relationship between oocytes and somatic cells have not been accurately resolved. Here, we performed scRNA-Seq, as well as spatial transcriptomic sequencing to profile the ovarian transcriptome atlas in rat. We identified four components of developing granulosa cell (GC) including cumulus, primitive, mural, and luteal cells and constructed their differential transcriptional regulatory networks. Several undiscovered growth signalings were identified from oocytes to cumulus cells, such as Jag1/Notch2 and Fgf9/ Fgfr2. Moreover, we described three cumulus sequential phases with follicle development and determined key transcriptional factors in each cumulus phases. We also pinpointed that macrophages may involve in luteal regression. Altogether, The single-cell spatial transcriptomic profile of the ovary provide a new research dimension for temporal and spatial analysis of ovarian development. It is also provided an important data resources and research basis for in-depth excavation of the mechanisms of mammalian ovary development. |
| 样品范围 |
Single cell |
| 发布日期 |
2024-02-01 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 37309718
|
A spatiotemporal gene expression and cell atlases of the developing rat ovary
|
Cell Proliferation
|
10.1111/cpr.13516
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| The National Key R&D Program of China
|
|
2019YFA0110900
|
|
| Strategic Priority Research Program of the Chinese Academy of Sciences
|
|
XDA16010501
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81820108016
|
|
| National Natural Science Foundation of China (NSFC)
|
|
32070848
|
|
|
|
| 提交者 |
yang
ying (yingyang@big.ac.cn)
|
| 提交单位 |
Beijing Institute of Genomics |
| 提交日期 |
2022-11-07 |
