| 项目编号 |
PRJCA013030 |
| 项目标题 |
Autophagy plays a role the maturation of hematopoietic precursors through nucleolin pathways in the embryo |
| 涉及领域 |
Model organism |
| 数据类型 |
Transcriptome or Gene expression
Single cell sequencing
|
| 物种名称 |
Mus musculus
|
| 描述信息 |
The generation of hematopoietic stem cell (HSC) in vitro is the powerful sources for treatment of blood disease. Understanding of regulatory mechanisms on HSC development in the embryo is able to facilitate achievement of this goal. AGM region is the site for HSC production in the embryo, through forming hematopoietic clusters from hemogenic endothelial cells. Distinct regulators are involved in the process, however, it is not yet known whether macroautophagy (autophagy) regulates hematopoietic development in the pre-liver stage of embryo. Here, by using the LC3-RFP-EGFP autophagy reporter mouse model, we present that different statuses of autophagy existed in the hematopoietic precursors, correlating with hematopoietic potential. Autophagy related gene (Atg) 5 deficiency specifically in endothelial cell disrupted endothelial cell to hematopoietic transition (EHT) via blocking the fusion between autophagosome and lysosome. Using combined approaches, including single cell RNA-sequencing (scRNA-seq), we confirmed that Atg5 deletion interrupted the developmental temporal order of EHT to further affect the pre-HSC I maturation. The rescue experiments with in vivo analysis suggest that autophagy influenced the hemogenic potential of endothelial cells via nucleolin pathways. These findings show that autophagy plays vital roles in the formation / maturation of hematopoietic precursors derived from hemogenic endothelial cells. |
| 样品范围 |
Single cell |
| 发布日期 |
2024-09-27 |
| 出版信息 |
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82070105
|
|
| National Key Research and Development Program
|
|
2019YFA0801802
|
|
| National Key Research and Development Program
|
|
2019YFA011-1100
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Research and Development Program of China
|
2019YFA0801800
|
|
|
|
| 提交者 |
zhuan
li (zhuanli2018@smu.edu.cn)
|
| 提交单位 |
Southern Medical University |
| 提交日期 |
2022-11-03 |
