| Accession |
PRJCA013030 |
| Title |
Autophagy plays a role the maturation of hematopoietic precursors through nucleolin pathways in the embryo |
| Relevance |
Model organism |
| Data types |
Transcriptome or Gene expression
Single cell sequencing
|
| Organisms |
Mus musculus
|
| Description |
The generation of hematopoietic stem cell (HSC) in vitro is the powerful sources for treatment of blood disease. Understanding of regulatory mechanisms on HSC development in the embryo is able to facilitate achievement of this goal. AGM region is the site for HSC production in the embryo, through forming hematopoietic clusters from hemogenic endothelial cells. Distinct regulators are involved in the process, however, it is not yet known whether macroautophagy (autophagy) regulates hematopoietic development in the pre-liver stage of embryo. Here, by using the LC3-RFP-EGFP autophagy reporter mouse model, we present that different statuses of autophagy existed in the hematopoietic precursors, correlating with hematopoietic potential. Autophagy related gene (Atg) 5 deficiency specifically in endothelial cell disrupted endothelial cell to hematopoietic transition (EHT) via blocking the fusion between autophagosome and lysosome. Using combined approaches, including single cell RNA-sequencing (scRNA-seq), we confirmed that Atg5 deletion interrupted the developmental temporal order of EHT to further affect the pre-HSC I maturation. The rescue experiments with in vivo analysis suggest that autophagy influenced the hemogenic potential of endothelial cells via nucleolin pathways. These findings show that autophagy plays vital roles in the formation / maturation of hematopoietic precursors derived from hemogenic endothelial cells. |
| Sample scope |
Single cell |
| Release date |
2024-09-27 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
|
|
Autophagy regulates the maturation of hematopoietic precursors in the embryo
|
Research Square
|
10.21203/rs.3.rs-3288232/v1
|
2023
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
82070105
|
|
| National Key Research and Development Program
|
|
2019YFA0801802
|
|
| National Key Research and Development Program
|
|
2019YFA011-1100
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Research and Development Program of China
|
2019YFA0801800
|
|
|
|
| Submitter |
zhuan
li (zhuanli2018@smu.edu.cn)
|
| Organization |
Southern Medical University |
| Submission date |
2022-11-03 |
