Accession

PRJCA013091

Title

Multi-omics profiling characterizes the distinct features of small extracellular vesicles derived from breast cancer tissues and 3D-culture models

Relevance

Medical

Data types

Raw sequence reads

Organisms

Homo sapiens

Description

Small extracellular vesicles (sEVs) are nanometric vesicles (less than 200 nm in diameter), enveloped by a lipid bi-layer and formed in vesicular bodies with the endosomal network. In this study, we isolated and characterized sEVs directly from fresh human breast cancer tissues (T-sEVs), paired normal tissues (N-sEVs) and corresponding plasma (B-sEVs), meanwhile we also collected sEVs from the paired tumor OTS (S-sEVs) and organoid (O-sEVs) models as well. By examining both transcriptomes and proteomes of sEVs from these various sources, we tried to identify the actual breast cancer-specific sEV signatures, and illuminate the key different features between sEVs derived from the fresh tissues and the commonly used in vitro models.

Sample scope

Multiisolate

Release date

2024-02-02

Publication

PubMed ID	Article title	Journal name	DOI	Year
38053815	Integrated transcriptomics, proteomics, and functional analysis to characterize the tissue‐specific small extracellular vesicle network of breast cancer	MedComm	10.1002/mco2.433	2023

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)		81773065
National Natural Science Foundation of China (NSFC)		81800024
National Natural Science Foundation of China (NSFC)		81502463
National Natural Science Foundation of China (NSFC)		82173089
Natural Science Foundation of Zhejiang Province		LR19H160001
Natural Science Foundation of Zhejiang Province		LY21H100004
Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang		2019R01007

Submitter

Chao Ni (davenc@163.com)

Organization

The Second Affiliated Hospital, Zhejiang University School of Medicine

Submission date

2022-11-09

Project Data

Resource name	Description
BioSample (41) show	-
SAMC2414632	Patient 10 sEVs from organoid
SAMC2414628	Patient 9 sEVs from organoid
SAMC2414624	Patient 8 sEVs from organoid
SAMC2414620	Patient 7 sEVs from organoid
SAMC2414616	Patient 2 sEVs from organoid
SAMC2414612	Patient 7 sEVs from normal tissue
SAMC2414608	Patient 7 sEVs from breast cancer tissue
SAMC2414604	Patient 7 sEVs from plasma
SAMC2414600	Patient 7 sEVs from organotypic tissue slices
SAMC2414596	Patient 6 sEVs from organoid
SAMC2414592	Patient 5 sEVs from organoid
SAMC2414588	Patient 4 sEVs from organoid
SAMC2414583	Patient 3 sEVs from organoid
SAMC2414578	Patient 10 sEVs from normal tissue
SAMC2414573	Patient 10 sEVs from breast cancer tissue
SAMC2414559	Patient 9 sEVs from normal tissue
SAMC2414554	Patient 9 sEVs from breast cancer tissue
SAMC2414550	Patient 8 sEVs from normal tissue
SAMC2414545	Patient 8 sEVs from breast cancer tissue
SAMC2414541	Patient 6 sEVs from normal tissue
SAMC2414537	Patient 6 sEVs from breast cancer tissue
SAMC2414534	Patient 5 sEVs from breast cancer tissue
SAMC2414530	Patient 4 sEVs from normal tissue
SAMC2414526	Patient 4 sEVs from breast cancer tissue
SAMC2414523	Patient 3 sEVs from normal tissue
SAMC2414519	Patient 3 sEVs from breast cancer tissue
SAMC2414515	Patient 2 sEVs from breast cancer tissue
SAMC2414511	Patient 10 sEVs from organotypic tissue slices
SAMC2414507	Patient 10 sEVs from plasma
SAMC2414502	Patient 9 sEVs from organotypic tissue slices
SAMC2414498	Patient 8 sEVs from plasma
SAMC2414494	Patient 8 sEVs from organotypic tissue slices
SAMC2414490	Patient 6 sEVs from plasma
SAMC2414486	Patient 6 sEVs from organotypic tissue slices
SAMC2414483	Patient 5 sEVs from plasma
SAMC2414479	Patient 4 sEVs from organotypic tissue slices
SAMC2414475	Patient 3 sEVs from organotypic tissue slices
SAMC2414472	Patient 2 sEVs from plasma
SAMC2414468	Patient 1 sEVs from plasma
SAMC2414464	Patient 1 sEVs from organotypic tissue slices
SAMC2414459	Patient 1 sEVs from breast cancer tissue