| 项目编号 |
PRJCA013173 |
| 项目标题 |
High-throughput Saturation Mutagenesis Generates a High-affinity Antibody against SARS-CoV-2 Variants using Protein Surface Display Assay on a Human Cell |
| 涉及领域 |
biomedicine |
| 数据类型 |
Transcriptome or Gene expression
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
As new mutations continue to emerge, the ability of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus to evade the human immune system and neutralizing antibodies remains a huge challenge for vaccine development and antibody research. The majority of neutralizing antibodies have reduced or lost activity against SARS-CoV-2 variants. In this study, we reported a novel protein surface display system on a mammalian cell for obtaining a higher-affinity antibody in high-throughput manner. Using a saturation mutagenesis strategy through integrating microarray-based oligonucleotide synthesis and single-cell screening assay, we generated a group of new antibodies against diverse prevalent SARS-CoV-2 variants through high-throughput screening the human antibody REGN10987 within 2 weeks. |
| 样品范围 |
Monoisolate |
| 发布日期 |
2023-03-25 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 36724179
|
High-throughput saturation mutagenesis generates a high-affinity antibody against SARS-CoV-2 variants using protein surface display assay on a human cell
|
PLoS Pathogens
|
10.1371/journal.ppat.1011119
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
81827809
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81421004
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82150005
|
|
| National Natural Science Foundation of China (NSFC)
|
|
T2288102
|
|
| National Key Basic Research Project of China
|
|
2018YFA0108101
|
|
| PKU-Baidu Fund
|
|
2020BD018
|
|
|
|
| 提交者 |
Jianzhong
Xi (jzxi@pku.edu.cn)
|
| 提交单位 |
Peking University |
| 提交日期 |
2022-11-13 |
