| Accession |
PRJCA013785 |
| Title |
High-throughput screening of spike variants uncover the key residues that alter affinity and antigenicity |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
Raw sequence reads
|
| Organisms |
Homo sapiens
|
| Description |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) elicited a worldwide pandemic since the late 2019. Till now, it has brought about 640 million confirmed cases, leading to more than 6.6 million deaths. Five variants of concern (VOCs) were tracked one after another and characterized subsequently. However, it is still hard to predict the next dominant lineage due to the rapid evolution of its spike (S) glycoprotein, which influenced the binding activity to cellular receptor angiotensin-converting enzyme 2 (ACE2) and drifted the presenting epitope to humoral immune response. Here, we established a robust mammalian cell-surface-display platform for studying the affinity between S and ACE2 in a large-scale manner. Lentivirus library of S variants was generated via in-silico chip synthesis following by site-directed saturation mutagenesis, and the enriched candidates were acquired through singe-cell fluorescence sorting and analyzed by the third-generation DNA sequencing technologies. The mutational landscape provides a blueprint for understanding the key residues of S anchor to ACE2. |
| Sample scope |
Monoisolate |
| Release date |
2022-12-09 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 37041132
|
High-throughput screening of spike variants uncovers the key residues that alter the affinity and antigenicity of SARS-CoV-2
|
Cell Discovery
|
10.1038/s41421-023-00534-2
|
2023
|
|
|
| Biomaterial provider |
Jianzhong Xi |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
National Key Basic Research Project of China
|
2018YFA0108101
|
N/A
|
| National Natural Science Foundation of China (NSFC)
|
National Natural Science Foundation of China
|
81827809
|
N/A
|
| National Natural Science Foundation of China (NSFC)
|
National Natural Science Foundation of China
|
82150005
|
N/A
|
| PKU-Baidu
|
PKU-Baidu Fund
|
2020BD018
|
N/A
|
| National Natural Science Foundation of China (NSFC)
|
National Key R&D Program of China
|
2021YFC2301700
|
N/A
|
|
|
| Submitter |
Jianzhong
Xi (jzxi@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2022-12-09 |
