| Description |
Human amnion (n=3), chorion (n=2), umbilical cord (n=3), and adipose (n=2) MSCs were obtained, as were human UCMSCs of P0 (n=2), P3 (n=2), P5 (n=2), and P5L (n=2) generations in normoxic and hypoxic culture. Including all, 18 samples were acquired, and scRNA-seq generated transcriptome information from 138,907 cells for downstream analysis. This study investigates transcriptional heterogeneity in MSCs from various tissue origins, generations, and culture conditions. MSCs from diverse tissue origins differ greatly in tissue repair, differentiation potential, proliferative capacity, and external vesicle transport, whereas MSCs from different generations and hypoxic cultures differ mostly in cell pluripotency. MSCs from neonatal and adult sources disclose MSC subpopulations and roles from many viewpoints, providing direction for clinical application of MSCs and supporting quality control improvement and clinical medication discovery for MSCs. |
