| 项目编号 |
PRJCA014157 |
| 项目标题 |
Single-cell RNA-seq combined spatial transcriptomics analysis decipher cell atlas of human ligament and reveal the process of ligament degeneration |
| 涉及领域 |
Medical |
| 数据类型 |
Single cell sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Anterior cruciate ligament degeneration is an important origin of knee osteoarthritis and a common threat to athletic ability. However, the cellular heterogeneity and underlying mechanisms in the degeneration process remain elusive. To systematically identify and localize cell compartments in normal and degenerated ligaments and investigate how cellular compositions, functions, and interactions participated in the ligamental degeneration, we built a cell atlas of the healthy and degenerated human ligament using scRNA-seq and spRNA-seq. We identified new fibroblast subgroups contributed to the disease and mapped out their spatial distribution in the tissue and revealed two stages of the degenerative process. We compared the cellular interactions between different tissue states and identified important signaling pathways may contribute to the disease. These findings will help us better understand anterior cruciate ligamental degeneration pathogenesis, and provide potential targets for ligamental injury therapies. |
| 样品范围 |
Single cell |
| 发布日期 |
2024-03-20 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
|
|
A single-cell atlas depicting the cellular and molecular features in human ligamental degeneration: a single cell combined spatial transcriptomics study
|
medRXiv
|
10.1101/2023.01.05.23284219
|
2023
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82172508
|
|
|
|
| 提交者 |
WEILI
FU (foxwin2008@163.com)
|
| 提交单位 |
West China Hospital of Sichuan University |
| 提交日期 |
2022-12-30 |
