| Accession |
PRJCA014280 |
| Title |
FACT Functions in Pluripotency State Transition of mESC |
| Relevance |
Model organism |
| Data types |
Raw sequence reads
ChIP-seq and RNA-seq
|
| Organisms |
Mus musculus
|
| Description |
mESCs with SSRP1-Q265K mutation showed increased spontaneous naive to formative phase transition, indicating the function of FACT in promoting cell fate decision. Surprisingly, the SSRP1-Q265K mutation increased formative gene expression and FACT binding at enhancers regulating formative genes, suggesting that FACT regulates transcription by regulating enhancers. Furthermore, this regulation potentially depends on eRNA transcription. In summary, this study proposes a novel mechanism that FACT controls formative gene expression through enhancers, which provides new insights into the functions of epigenetic regulation in pluripotency control during embryonic development. |
| Sample scope |
Multiisolate |
| Release date |
2024-01-22 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
31725015
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31830048
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31861143041
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31671332
|
|
| Beijing Outstanding Young Scientist Program
|
|
BJJWZYJH01201910001005
|
|
| National Key Research and Development Project of China
|
|
2019YFA0508903
|
|
| Funding for open access charge: Beijing Outstanding Young Scientist Program
|
|
BJJWZYJH01201910001005
|
|
| National Natural Science Foundation of China, Youth Program
|
|
31900433
|
|
|
|
| Submitter |
Qing
Li (li.qing@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2023-01-06 |
