| 项目编号 | PRJCA014395 | ||||||||
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| 项目标题 | Targeted Next-Generation Sequencing with MultiPrime: A Reliable and Efficient Tool for Global Pathogen Detection. | ||||||||
| 涉及领域 | Medical | ||||||||
| 数据类型 |
Metagenome
Targeted loci cultured |
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| 物种名称 |
Human respirovirus 3
Human alphaherpesvirus 3 Rotavirus unidentified human coronavirus Lyssavirus rabies SFTS phlebovirus Enterovirus Human orthopneumovirus Seoul orthohantavirus Haemophilus influenzae Pestivirus C Streptococcus pneumoniae Parechovirus A Simian T-lymphotropic virus 3 Japanese encephalitis virus unidentified influenza virus Human metapneumovirus |
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| 描述信息 | Targeted next-generation sequencing (tNGS) offers a cost-effective option for pathogen diagnostics and therapeutics. Nevertheless, the design of multiplex primers constrains its wide-spectrum target coverage. Herein, we present multiPrime, which enables a more extensive coverage of pathogen spectra while maintaining high multiplexing compatibility. Our study demonstrates that multiPrime outperforms DegePrime with fewer primers and greater coverage on 8 viruses. Furthermore, multiPrime-designed primers successfully amplified all target amplicons across these viruses. We utilized multiPrime to design primers for a tNGS panel that covers 30 viruses, validating the panel's performance in 80 clinical specimens, achieving 94% sensitivity and 89% specificity. | ||||||||
| 样品范围 | Multispecies | ||||||||
| 发布日期 | 2025-02-13 | ||||||||
| 项目资金来源 |
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| 提交者 | Junbo Yang (1806389316@pku.edu.cn) | ||||||||
| 提交单位 | hugo biotech | ||||||||
| 提交日期 | 2023-01-12 |