| Accession |
PRJCA014524 |
| Title |
Rapid detection of isocitrate dehydrogenase 1 mutation status in glioma based on Crispr-Cas12a |
| Relevance |
Medical |
| Data types |
Phenotype or Genotype
|
| Organisms |
Homo sapiens
|
| Description |
The aim is to use Crispr-Cas12a for the rapid detection of the single nucleotide polymorphism (SNP) of isocitrate dehydrogenase 1 (IDH1)-R132H locus and explore the effectiveness and consistency of this method with direct sequencing method for detecting IDH1-R132H of glioma tissue samples. 58 previous frozen tissue and 46 recent fresh tissue samples of adult diffuse glioma were selected to detect IDH1-R132H using Crispr-Cas12a. The results of immunohistochemistry (IHC) and direct sequencing methods were analyzed. |
| Sample scope |
Multiisolate |
| Release date |
2023-01-19 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 37029174
|
Rapid detection of isocitrate dehydrogenase 1 mutation status in glioma based on Crispr-Cas12a
|
Scientific Reports
|
10.1038/s41598-023-32957-y
|
2023
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| No funding support
|
|
|
|
|
|
| Submitter |
shiyu
feng (fsy72123@163.com)
|
| Organization |
Chinese PLA General Hospital |
| Submission date |
2023-01-19 |
