| Accession |
PRJCA015597 |
| Title |
Multi-scale analysis of chromatin high-order structure and regulation |
| Relevance |
Model organism |
| Data types |
Cryo-em structure
|
| Organisms |
Escherichia coli
Homo sapiens
|
| Description |
During the development and differentiation of individuals in higher organisms, cell fate is determined mainly by selective gene silencing or gene activation through epigenetic mechanisms, while epigenetic regulation is achieved mainly by regulating dynamic changes in the chromatin high-order structure. An important biological question after the completion of the Human Genome Project is: How are the fates of various cells with different functions in the same individual with the same genome determined and differentiated? The structural and dynamic changes in chromatin play a key role. Organisms determine the tissue specificity and fate of cells by regulating the dynamics of chromatin high-level structure and thus the level of gene transcription. This topic uses in vitro chromatin assembly system to reconstruct 30 nm chromatin fibers, and integrates multi-scale technical means such as analytical ultracentrifugation, high-precision single-particle 3D reconstruction, and electron tomography imaging to study the structure of 30 nm chromatin fibers and elucidate the molecular mechanisms of chromatin high-order structure establishment, maintenance and its dynamic regulation. |
| Sample scope |
structure |
| Release date |
2023-04-03 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Chinese Academy of Sciences (CAS)
|
|
XDB37000000
|
|
|
|
| Submitter |
Xueli
Zhang (zhangxueli@ibp.ac.cn)
|
| Organization |
Institute of Biophysics, Chinese Academy of Sciences |
| Submission date |
2023-03-16 |
