Accession PRJCA015823
Title whole genome resequencing of E. coli
Relevance Evolution
Data types Raw sequence reads
Organisms Escherichia coli
Description whole genome resequencing of E. coli (100 strains). (1) 1-45 strains: Genomes were paired-end re-sequenced using the Illumina HiSeq 2000/2500 instrument (Illumina inc., San Diego, CA, USA) with around 20 coverage for each genome. The initial strains in this experiment were genomes sequenced. Genomic DNA from different isolates was all genomes sequenced independently. Reads were 100/125 bp long, sequenced in pairs with a mean insert size of 500 bp. For E. coli between 405 and 1,260 Mb clean data (793 Mb on average) were obtained for each individual. (2) 46-100 strains: Genomes were paired-end re-sequenced using the Illumina HiSeq 4000 instrument (Illumina inc., San Diego, CA, USA) with around 20 coverage for each genome. The initial strains in this experiment were genomes sequenced. Genomic DNA from different isolates was all genomes sequenced independently. Reads were 150 bp long, sequenced in pairs with a mean insert size of 300 bp. For E. coli between 727 and 1,268 Mb clean data (921 Mb on average) were obtained for each individual.
Sample scope Multiisolate
Release date 2023-03-31
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 31971398
External link
External link Link description
https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA319751 NCBI: 45 strains E. coli (PRJNA319751)
https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA550335 NCBI: 55 strains E. coli (PRJNA550335)
Submitter Caifeng Li (cfli2020@bjfu.edu.cn)
Organization Beijing Forestry University
Submission date 2023-03-25

Project Data

Resource name Description
BioSample (100)  show -
GSA (1) -
CRA010512 whole genome resequencing of E. coli