项目编号

PRJCA015861

项目标题

Single-cell Transcriptomic Analysis Reveals Rich Pituitary-immune Interactions under Systemic Inflammation

涉及领域

Medical

数据类型

Transcriptome or Gene expression
Raw sequence reads
Single cell sequencing

物种名称

Mus musculus

描述信息

The pituitary represents an essential hub in the hypothalamus-pituitary-adrenal (HPA) axis. Pituitary hormone-producing cells (HPCs) release several hormones to regulate fundamental bodily functions under normal and stress conditions. It is well established that the pituitary endocrine gland modulates the immune system by releasing adrenocorticotropic hormone (ACTH) in response to neuronal activation in the hypothalamus. However, it remains unclear how systemic inflammation regulates the transcriptomic profiles of pituitary HPCs. Here, we performed single-cell RNA- sequencing (scRNA-seq) of the pituitary and revealed that upon inflammation, all major pituitary HPCs respond robustly in a cell type-specific manner, with corticotropes displaying the strongest reaction. Systemic inflammation also led to the production and release of noncanonical bioactive molecules, including NPTX2 by corticotropes, to modulate immune homeostasis. Meanwhile, HPCs upregulate the gene expression of chemokines that facilitate the communication between the HPCs and immune cells. Together, our study reveals extensive interactions between the pituitary and immune system, suggesting the roles of the pituitary in mediating the effects of inflammation on many aspects of body physiology.

样品范围

Single cell

发布日期

2023-10-10

项目资金来源

机构	项目类型	授权项目ID	授权项目名称
Ministry of Science and Technology of the People's Republic of China (MOST)		2021ZD0202803
Research Unit of Medical Neurobiology at Chinese Academy of Medical Sciences		2019RU003

提交者

Ruiyu Wang (wangruiyu@cibr.ac.cn)

提交单位

Chinese Institute for Brain Research

提交日期

2023-03-27

项目包含数据信息

资源名称	描述
BioSample (74) show	-
SAMC1176312	RNA-seq of mus musculus: pituitary LPS
SAMC1176311	RNA-seq of mus musculus: pituitary LPS
SAMC1176310	RNA-seq of mus musculus: pituitary LPS
SAMC1176309	RNA-seq of mus musculus: pituitary CLP model
SAMC1176308	RNA-seq of mus musculus: pituitary CLP model
SAMC1176307	RNA-seq of mus musculus: pituitary CLP model
SAMC1176306	RNA-seq of mus musculus: pituitary saline
SAMC1176305	RNA-seq of mus musculus: pituitary saline
SAMC1176304	RNA-seq of mus musculus: pituitary saline
SAMC1176303	RNA-seq of mus musculus: pituitary ctrl
SAMC1176302	RNA-seq of mus musculus: pituitary ctrl
SAMC1176301	RNA-seq of mus musculus: pituitary ctrl
SAMC1176300	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176299	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176298	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176297	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176296	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176295	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176294	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176293	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176292	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176291	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176290	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176289	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176288	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176287	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176286	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176285	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176284	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176283	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176282	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176281	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176280	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176279	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176278	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176277	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176276	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176275	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176274	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176273	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176272	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176271	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176270	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176269	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176268	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176267	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176266	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176265	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176264	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176263	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176262	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176261	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176260	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176259	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176258	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176257	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176256	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176255	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176254	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176253	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176252	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176251	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176250	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176249	scRNA-seq of mus musculus: pituitary inflammatory
SAMC1176248	scRNA-seq of mus musculus: pituitary healthy
SAMC1176247	scRNA-seq of mus musculus: pituitary healthy
SAMC1176246	scRNA-seq of mus musculus: pituitary healthy
SAMC1176245	scRNA-seq of mus musculus: pituitary healthy
SAMC1176244	scRNA-seq of mus musculus: pituitary healthy
SAMC1176243	scRNA-seq of mus musculus: pituitary healthy
SAMC1176242	scRNA-seq of mus musculus: pituitary healthy
SAMC1176241	scRNA-seq of mus musculus: pituitary healthy
SAMC1176240	scRNA-seq of mus musculus: pituitary healthy
SAMC1176239	scRNA-seq of mus musculus: pituitary healthy
GSA (1)	-
CRA010480	Single-cell Transcriptomic Analysis Reveals Rich Pituitary-immune Interactions under Systemic Inflammation