项目编号 PRJCA015888
项目标题 Two haplotype-resolved genomes of highly heterozygous AAB allotriploid bananas provide insights into subgenome asymmetric evolution and banana wilt control
涉及领域 Agricultural
数据类型 Whole genome sequencing
Transcriptome or Gene expression
Genome sequencing and assembly
Raw sequence reads
Genome sequencing
Assembly
物种名称 Musa x paradisiaca
Musa AAB Group
描述信息 Bananas (Musa spp.) are one of the most important tropical fruits and staple food, which are of great significance to human societies. Plantain and Silk are two important banana subgroups, which are both triploid hybrids (AAB) between the wild diploid Musa acuminata and M. balbisiana. In this study, we reported the first haplotype-resolved genome assembly of Plantain and Silk bananas with genome sizes of approximately 1.4 Gb. We discovered widespread asymmetric evolution in the subgenomes of Plantain and Silk, which could be linked to frequent homologous exchanges (HEs) events. This is the first study to uncover the genetic makeup of triploid banana and verify that subgenome B harbors a rich source of resistance genes. Of the 88,078 and 94,988 annotated genes in Plantain and Silk, only 58.5% and 59.4% were present in all three subgenomes, with >50% genes containing differently expressed alleles in different haplotypes. We also found that Plantain is more resistant to banana Fusarium wilt, exhibiting a much faster defense response after pathogenic fungi infection. A large number of differentially expressed genes in abscisic acid, ethylene, jasmonic acid and salicylic acid pathways were identified in Plantain. Our analysis revealed that MpMYB36 promotes the biosynthesis of secondary cell wall and deposition of lignin by directly binding to the promoter of MpPAL and MpHCT, which allows Plantain to inhibit the penetration of early infection. Moreover, the insertion of the key carotenoid synthesis gene (CRTISO) may be the potential genetic basis for the richness of carotenoids in Plantain. Our study provides an unprecedented genomic basis for basic research and the development of elite germplasm in cultivated bananas.
样品范围 Multispecies
发布日期 2023-12-07
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Ministry of Science and Technology of the People's Republic of China (MOST) National Key R&D Program of China 2019YFD1000203
提交者 wenzhao Xie (xwz080311@163.com)
提交单位 Huazhong Agricultural University
提交日期 2023-03-28

项目包含数据信息

资源名称 描述
BioSample (178)  show -
GSA (1) -
CRA010481 Raw data of Musa spp. Plantain AAB subgroup cv 'Batard' and Musa spp. Silk AAB subgroup cv 'Figue Pomme Geante' (ITC0769)