| Description |
Studying the structure and function of biomolecular complexes in cells is an important way to reveal the laws of life, understand the essence of life, and map the blueprint of life. The coherent X-ray diffraction imaging technology developed by the applicant in recent years has overcome the limitations of traditional imaging techniques due to optical component constraints and theoretically can achieve diffraction-limited resolution. This technology has significant advantages in imaging contrast and quantitative imaging of material density, and is suitable for the study of the microscopic structure of samples such as cells and tissues. In order to achieve precise measurement and characterization of biomolecular complexes in cells, this project focuses on the significant need for cross-scale structural research on biomolecular complexes. Building on the preliminary research of the applicant's team, the following four technical research areas are proposed: 1) Developing and improving an in-situ, three-dimensional high-resolution, and quantitative multiscale X-ray imaging system based on third-generation synchrotron radiation sources, achieving imaging resolution better than 10nm for cells; 2) Establishing, improving, and applying high-resolution and multimodal imaging methods to explore the integrated imaging of the structure and function of biomolecular complexes in situ distribution in cells/tissues; 3) Developing low-dose and multiscale X-ray nanoscale imaging to address radiation damage issues in biological imaging; 4) Using advanced X-ray free-electron laser devices to explore the in-situ, high-resolution, and non-destructive imaging of biomolecular complexes in living cells, and developing cutting-edge X-ray imaging technologies. Through this research, innovative and applied technology methods for in-situ structural quantitative analysis and cross-scale analysis will be developed, providing technical support for the study of ultra-large biomolecular complexes and promoting the application of new generation X-ray imaging technology in life science. |
