| 项目编号 | PRJCA016057 | ||||||||
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| 项目标题 | The essential role of RNA polymerase I transcription in establishing the chromatin architecture in mouse embryos | ||||||||
| 涉及领域 | Medical | ||||||||
| 数据类型 |
Epigenomics
Raw sequence reads Genome sequencing |
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| 物种名称 |
Homo sapiens
Mus |
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| 描述信息 | The chromatin structure in mice undergoes drastic reprogramming during gametogenesis and early embryonic development. However, differences in the three-dimensional chromatin structure between humans and mice as well as the molecular mechanisms driving de novo organization are poorly understood. Here, we found global three-dimensional genome reorganization from human oogenesis to early embryonic development, and different characteristics and mechanisms from those in mice. Furthermore, integrative analysis by clustered regularly interspaced short palindromic repeat/Cas13a gene editing, low-input genome-wide chromosome conformation capture, cleavage under targets and tagmentation, and RNA-sequencing showed that RNA polymerase I (Pol I) was essential for the de novo chromatin structure during mouse embryonic development. Mouse embryos were blocked at the 2-cell phase after knockout or inhibition of Pol I, and the biological processes related to chromatin silencing was enriched. More importantly, inhibition of Pol I transcription prevented establishment of the TAD structure in mouse embryos, but not in human embryos. Additionally, the TAD structure was weakened in mouse female germline stem cells lacking Pol I transcription, but was enhanced in human female germline stem cells. These findings provide a valuable resource for future studies of chromatin organization during germ cell and embryonic development, and are of great significance to maintain fertility and prevent birth defects. | ||||||||
| 样品范围 | Multispecies | ||||||||
| 发布日期 | 2025-04-11 | ||||||||
| 项目资金来源 |
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| 提交者 | Ji Wu (jiwu@sjtu.edu.cn) | ||||||||
| 提交单位 | Shanghai Jiao Tong University | ||||||||
| 提交日期 | 2023-04-04 |