Accession PRJCA016271
Title Single-cell Multi-omics Analysis of Human Testicular Germ Cell Tumor Reveals its Molecular Features and Microenvironment
Relevance Medical
Data types Raw sequence reads
Single cell sequencing
Organisms Homo sapiens
Description Seminoma is the most common malignant solid tumor in 14 to 44 year-old men. However, its molecular features and tumor microenvironment (TME) is largely unexplored. Here, we performed a series of studies via genomics profiling (single cell multi-omics and spatial transcriptomics) and functional examination using seminoma samples and a seminoma cell line. We identified key gene expression programs shared between seminoma and primordial germ cells, and further characterized the functions of TFAP2C in promoting tumor invasion and migration. We also identified 15 immune cell subtypes in TME, and found that subtypes with exhaustion features were located closer to the tumor region through combined spatial transcriptome analysis. Furthermore, we identified key pathways and genes that may facilitate seminoma disseminating beyond the seminiferous tubules. These findings advanced our knowledge of seminoma tumorigenesis and produced a multi-omics atlas of in situ human seminoma microenvironment, which could help discover potential therapy targets for seminoma.
Sample scope Single cell
Release date 2023-12-05
Publication
PubMed ID Article title Journal name DOI Year
38123589 Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment Nature Communications 10.1038/s41467-023-44305-9 2023
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) 2022YFC2702600
China Postdoctoral Science Foundation 2022M713135
Natural Science Foundation of Hunan Province 2021JJ41091
Submitter Jingtao Guo (jingtao.guo@ioz.ac.cn)
Organization Institute of Zoology, Chinese Academy of Sciences
Submission date 2023-04-15

Project Data

Resource name Description
BioSample (9)  show -