| 项目编号 |
PRJCA017167 |
| 项目标题 |
Distinct temporal patterns of super-enhancers reveal core regulatory network for developmental skeletal muscle of pig |
| 涉及领域 |
Agricultural |
| 数据类型 |
Epigenomics
Raw sequence reads
|
| 物种名称 |
Sus scrofa
|
| 描述信息 |
As the most abundant tissue in the body, skeletal muscle plays a crucial role in performing essential bodily functions. Pigs, being excellent biomedical models, are particularly valuable for studying skeletal muscle development due to the ease of obtaining sufficient skeletal muscle samples at various prenatal and postnatal developmental stages, as well as individuals with essentially similar genetic backgrounds. However, the dynamic landscape of super-enhancers (SEs) and their regulatory networks during skeletal muscle development remains unclear. This study aims to elucidate how SE-mediated transcriptional regulatory mechanisms drive pig skeletal muscle (PSM) development. To achieve this, we employed the Cleavage Under Targets and Tagmentation (CUT&Tag) technique to profile the H3K27ac landscapes and identify SEs in PSM samples from five developmental stages. These stages encompassed prenatal myofiber formation to postnatal myofiber hypertrophy. Through our analysis, we discovered a subset of SEs that significantly contribute to muscle development and traits associated with pig meat and carcass. Using an innovative approach that integrates information from eRNA annotation, GWAS signals, and high-throughput capture Self-transcribing Active Regulatory Region Sequencing (STARR-seq) experiments, we present potential critical enhancers and their regulatory networks that might potentially play important roles in skeletal muscle development and growth in mammals. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2024-07-01 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 39433439
|
Analyzing super-enhancer temporal dynamics reveals potential critical enhancers and their gene regulatory networks underlying skeletal muscle development
|
Genome Research
|
10.1101/gr.278344.123
|
2024
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
31902124
|
|
| Shenzhen Science and Technology Innovation Commission (SZSTI)
|
|
JCYJ20190814163803664
|
|
|
|
| 提交者 |
Zhang
Song (zhangsong0001@163.com)
|
| 提交单位 |
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences |
| 提交日期 |
2023-05-21 |
