| 项目编号 |
PRJCA017213 |
| 项目标题 |
A spatially resolved atlas of human breast cancer characterizes a pro-inflammatory vascular niche orchestrated by venular endothelial and smooth muscle cells |
| 涉及领域 |
Medical |
| 数据类型 |
Single cell sequencing
spatial transcriptome sequencing
|
| 物种名称 |
Homo sapiens
|
| 描述信息 |
Breast cancers present intricate microenvironments comprising heterotypic interactions between different cells. A global map outlining the cellular organization of breast cancer has yet to be established. Here, we leverage the DNA nanoball-based genome-wide in situ sequencing (Stereo-seq) to visualize geospatial architecture of thirty primary breast tumors and metastatic lymph nodes. The unprecedented single-cell resolution mapping enables accurate localization of various cell types within the tumor ecosystem and discerns the delicate structure of sporadic vasculature. In particular, we unveil the immunomodulatory function of venules, in which ACKR1+ venular endothelial cells and CCL21+ venular smooth muscle cells constitute a pro-inflammatory niche to synergistically promote immune cell chemotaxis and extravasation into tumors. High numbers of venules are associated with more immune infiltration and better clinical outcome of patients. This study represents currently the most comprehensive survey of the spatially resolved atlas of human breast cancer across four molecular subtypes and provides critical insights into the phenotypic and spatial heterogeneity of tumor vasculature. |
| 样品范围 |
Multiisolate |
| 发布日期 |
2025-02-11 |
| 出版信息 |
| PubMed ID |
文章标题 |
杂志名称 |
Doi |
发表年份 |
| 40199901
|
Spatially resolved atlas of breast cancer uncovers intercellular machinery of venular niche governing lymphocyte extravasation
|
Nature Communications
|
10.1038/s41467-025-58511-0
|
2025
|
|
|
AF9/KLF2 gene regulatory circuit links histone lactylation to breast cancer metastasis
|
BioRxiv
|
10.1101/2025.06.18.660498
|
2025
|
|
|
| 项目资金来源 |
| 机构 |
项目类型 |
授权项目ID |
授权项目名称 |
| National Natural Science Foundation of China (NSFC)
|
|
82072097
|
|
| National Natural Science Foundation of China (NSFC)
|
|
82172876
|
|
| National Natural Science Foundation of China (NSFC)
|
|
81700436
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2021-1-I2M-012
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2021-I2M-1-014
|
|
| CAMS Innovation Fund for Medical Sciences
|
|
2022-I2M-C&T-B-078
|
|
| Beijing Hope Run Special Fund of Cancer Foundation of China
|
|
LC2020A18
|
|
| Scientific research topic of breast cancer single disease diagnosis and treatment ability improvement project
|
|
RXDBZ-2022-11
|
|
|
|
| 提交者 |
Nan
Sun (sunnan@cicams.ac.cn)
|
| 提交单位 |
Cancer Hospital, Chinese Academy of Medical Sciences |
| 提交日期 |
2023-05-24 |
