项目编号

PRJCA017220

项目标题

Metabolomics and Metagenomics Reveal the Impact of γδ T Inhibition on Gut Microbiota and Metabolism in Periodontitis-Promoting OSCC

涉及领域

Medical

数据类型

Metagenome
Genome sequencing
Metabolomics

物种名称

Microbiota
Mus

描述信息

In this study, a mouse model of OSCC with periodontitis was established, and γδ T cells were inhibited by antibodies. Gut samples from the mice were collected and analyzed by metabolomics, metagenomics, and 16S rRNA. Integrative analysis of the gut metabolome and microbiome revealed that targeting γδ T resulted in changes in the levels of metabolites associated with cancer in the gut. Although there was no difference in the α diversity of microbiota, β diversity was significantly different, with a more heterogeneous community structure in the mice receiving targeted γδ T immunotherapy. Statistical analysis of the gut microbiota at the species level revealed a significant enrichment of Lactobacillus murinus, which was significantly correlated with γδ T abundance. The functional analysis revealed that inhibiting γδ T could impact the functional gene. A comprehensive analysis revealed that Lactobacillus murinus is especially associated with changes in Adenine, who also had connection with the concentration of IL-17 and the abundance of γδ T.

样品范围

Synthetic

发布日期

2023-12-28

出版信息

PubMed ID	文章标题	杂志名称	Doi	发表年份
38259106	Metabolomics and metagenomics reveal the impact of γδ T inhibition on gut microbiota and metabolism in periodontitis-promoting OSCC	Computational Biology	10.1128/msystems.00777-23	2024

项目资金来源

机构	项目类型	授权项目ID	授权项目名称
National Natural Science Foundation of China (NSFC)		81991500
National Natural Science Foundation of China (NSFC)		81991502
Key Projects of Sichuan Provincial Department of Science and Technology		2020YFSY0008

提交者

jing li (2598295969@qq.com)

提交单位

Sichuan University

提交日期

2023-05-24

项目包含数据信息

资源名称	描述
BioSample (20) show	-
SAMC2865322	Metagenome sequencing of OSCC.P.GDT.5
SAMC2865321	Metagenome sequencing of OSCC.P.GDT.4
SAMC2865320	Metagenome sequencing of OSCC.P.GDT.3
SAMC2865319	Metagenome sequencing of OSCC.P.GDT.2
SAMC2865318	Metagenome sequencing of OSCC.P.GDT.1
SAMC2865317	Metagenome sequencing of OSCC.P.5
SAMC2865316	Metagenome sequencing of OSCC.P.4
SAMC2865315	Metagenome sequencing of OSCC.P.3
SAMC2865314	Metagenome sequencing of OSCC.P.2
SAMC2865313	Metagenome sequencing of OSCC.P.1
SAMC1269003	16S rRNA sequencing of OSCC.P.GDT.5
SAMC1269002	16S rRNA sequencing of OSCC.P.GDT.4
SAMC1269001	16S rRNA sequencing of OSCC.P.GDT.3
SAMC1269000	16S rRNA sequencing of OSCC.P.GDT.2
SAMC1268999	16S rRNA sequencing of OSCC.P.GDT.1
SAMC1268998	16S rRNA sequencing of OSCC.P.1
SAMC1268997	16S rRNA sequencing of OSCC.P.1
SAMC1268996	16S rRNA sequencing of OSCC.P.1
SAMC1268995	16S rRNA sequencing of OSCC.P.1
SAMC1268994	16S rRNA sequencing of OSCC.P.1
GSA (2)	-
CRA011605	Metagenome sequencing of gut microbiota in oral squamous cell carcinoma mice with periodontitis (or after gdt cell suppression)
CRA011276	16s RNA sequencing of intestinal flora of mice with oral squamous cell carcinoma of periodontitis (or after inhibiting gdt cells )