Accession

PRJCA017220

Title

Metabolomics and Metagenomics Reveal the Impact of γδ T Inhibition on Gut Microbiota and Metabolism in Periodontitis-Promoting OSCC

Relevance

Medical

Data types

Metagenome
Genome sequencing
Metabolomics

Organisms

Microbiota
Mus

Description

In this study, a mouse model of OSCC with periodontitis was established, and γδ T cells were inhibited by antibodies. Gut samples from the mice were collected and analyzed by metabolomics, metagenomics, and 16S rRNA. Integrative analysis of the gut metabolome and microbiome revealed that targeting γδ T resulted in changes in the levels of metabolites associated with cancer in the gut. Although there was no difference in the α diversity of microbiota, β diversity was significantly different, with a more heterogeneous community structure in the mice receiving targeted γδ T immunotherapy. Statistical analysis of the gut microbiota at the species level revealed a significant enrichment of Lactobacillus murinus, which was significantly correlated with γδ T abundance. The functional analysis revealed that inhibiting γδ T could impact the functional gene. A comprehensive analysis revealed that Lactobacillus murinus is especially associated with changes in Adenine, who also had connection with the concentration of IL-17 and the abundance of γδ T.

Sample scope

Synthetic

Release date

2023-12-28

Publication

PubMed ID	Article title	Journal name	DOI	Year
38259106	Metabolomics and metagenomics reveal the impact of γδ T inhibition on gut microbiota and metabolism in periodontitis-promoting OSCC	Computational Biology	10.1128/msystems.00777-23	2024

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)		81991500
National Natural Science Foundation of China (NSFC)		81991502
Key Projects of Sichuan Provincial Department of Science and Technology		2020YFSY0008

Submitter

jing li (2598295969@qq.com)

Organization

Sichuan University

Submission date

2023-05-24

Project Data

Resource name	Description
BioSample (20) show	-
SAMC2865322	Metagenome sequencing of OSCC.P.GDT.5
SAMC2865321	Metagenome sequencing of OSCC.P.GDT.4
SAMC2865320	Metagenome sequencing of OSCC.P.GDT.3
SAMC2865319	Metagenome sequencing of OSCC.P.GDT.2
SAMC2865318	Metagenome sequencing of OSCC.P.GDT.1
SAMC2865317	Metagenome sequencing of OSCC.P.5
SAMC2865316	Metagenome sequencing of OSCC.P.4
SAMC2865315	Metagenome sequencing of OSCC.P.3
SAMC2865314	Metagenome sequencing of OSCC.P.2
SAMC2865313	Metagenome sequencing of OSCC.P.1
SAMC1269003	16S rRNA sequencing of OSCC.P.GDT.5
SAMC1269002	16S rRNA sequencing of OSCC.P.GDT.4
SAMC1269001	16S rRNA sequencing of OSCC.P.GDT.3
SAMC1269000	16S rRNA sequencing of OSCC.P.GDT.2
SAMC1268999	16S rRNA sequencing of OSCC.P.GDT.1
SAMC1268998	16S rRNA sequencing of OSCC.P.1
SAMC1268997	16S rRNA sequencing of OSCC.P.1
SAMC1268996	16S rRNA sequencing of OSCC.P.1
SAMC1268995	16S rRNA sequencing of OSCC.P.1
SAMC1268994	16S rRNA sequencing of OSCC.P.1
GSA (2)	-
CRA011605	Metagenome sequencing of gut microbiota in oral squamous cell carcinoma mice with periodontitis (or after gdt cell suppression)
CRA011276	16s RNA sequencing of intestinal flora of mice with oral squamous cell carcinoma of periodontitis (or after inhibiting gdt cells )